In-silico analysis of WRKY Transcription Factors gene family in healthy and malformed stages of mango (Mangifera indica)

ASHOK YADAV; K USHA; PAWAN KUMAR JAYASWAL

doi:10.56093/ijas.v89i1.86188

Authors

ASHOK YADAV
K USHA
PAWAN KUMAR JAYASWAL

https://doi.org/10.56093/ijas.v89i1.86188

Keywords:

Cis-regulatory element, Defense, In-silico, Motif, Orthologs, Phylogenetic

Abstract

WRKY proteins play crucial roles in plant defense regulatory networks, development process and physiological programs including responses to several biotic and abiotic stresses. Evalutionary analysis revealed, WRKY genes were categorized into the four major groups. In developed phylogenetic tree, group-D contain highest number (15) of WRKY genes followed by group-B (10), group-A (7), and group-C (6). Several number of CREâ€™s were identified from mango transcriptome belonging to different categories like light responsiveness, hormone responsive, biotic
stress responsive, biotic stress responsive, binding, plant development, transcription and circadian control. Among the
10 stable genes observed in transcriptome, nine genes had negative Z-score indicating that these structures identified
for the proteins are reliable. Motif analysis indicated that the per cent occurrence of all the five motifs were higher in WRKY genes of malformed tissues compared to WRKY genes of healthy tissues. The uniquely identified CREâ€™s (Healthy stages: AC-II, GCC box, OBP; Malformed stages: Aux-RR-core, AC-I, 3-AF1 binding site, CAT-box, MNF1 and rbcS-CMA7a.), defense and stress responsiveness (TC-rich repeats) and fungal elicitor (Box-W1) related cis-regulatory elements will provide insight to solve the problem of mango malformation. The identified information regarding the WRKY Transcription Factor from mango transcriptome will serve as a valuable information for mango breeding against malformation.

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Author Biographies

ASHOK YADAV

Scientist, ICAR-Central Institute for Subtropical Horticulture, Regional Research Station, Malda, West Bengal 732 102.
K USHA

Division of Fruits and Horticultural
Technology, ICAR-IARI, New Delhi.
PAWAN KUMAR JAYASWAL

National Research Centre on Plant Biotechnology, New Delhi 110 012.

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