Evaluation of growth, nitrogen fixation and P-solubilizing ability of diazotrophic cyanobacteria under mineral phosphorus sources


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Authors

  • AMAN JAISWAL Ph D Scholar, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
  • RAHUL MISHRA Ph D Scholar, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
  • DEEPAK KUMAR KOLI Ph D Scholar, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
  • V K SHARMA Principal Scientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
  • SUNIL PABBI Principal Scientist, CCUBGA

https://doi.org/10.56093/ijas.v89i3.87581

Keywords:

AR activity, Available P, Cyanobacteria, Mussorie rock phosphate, Total chlorophyll, Tricalcium phosphate

Abstract

Five cyanobacterial strains, viz. Anabaena variabilis, Nostoc muscorum, Tolypothrix tenuis, Aulosira fertilissima and Westiellopsis prolifica were utilized extracellular insoluble tricalcium phosphate (TCP) and Mussorie rock phosphate (MRP) when provided as sole P source by replacing the conventional source of P (K2HPO4) in BG-11 medium. These strains exhibited a differential response to insoluble forms of phosphorus both in terms of growth and nitrogen fixation. A. variabilis, N. muscorum and W. proliï¬ca showed better growth in presence of TCP while T. tenuis and A. fertilissima proliferated better in presence of MRP. N. muscorum recorded maximum growth in terms of total chlorophyll content (6.21 μg/ml) in presence of TCP (20 mg P/l) followed by T. tenuis (6.10 μg/ml) in presence of MRP (20 mg P/l) after 21 days of incubation. Nitrogen fixation measured as acetylene reduction (AR) activity showed signiï¬cant variation among different strains when grown at varied concentrations of TCP or MRP and time of incubation. N. muscorum showed maximum AR activity of 6.17 μmole C2H4/mg chl/h in presence of MRP (20 mg P/l) followed by A. variabilis which recorded 5.46 μmole C2H4/mg chl/h but in presence of TCP (20 mg P/l) after 28 days of incubation. In general, in all the strains tested, AR activity was more in presence of higher concentration of TCP/ MRP than their lower concentrations (10 mg P/l). In terms of P-solubilization, A. variabilis was found to be the best, showing maximum amount of available P, i.e. 0.224 μg/ml and TCP (20 mg P/l) was the best utilized source of mineral P compared to MRP.

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References

Bardiya M C and Gaur A C.1972. Rock phosphate dissolution by bacteria. Indian Journal of Microbiology 12: 269–71.

Bbalali S, Hoseini S A, Ghorbani R and Kordi H. 2013. Relationships between nutrients and chlorophyll a concentration in the International Alma Gol Wetland, Iran. Journal of Aquaculture and Research Development 4(3): 68–75. DOI: https://doi.org/10.4172/2155-9546.1000173

Degerholm J, Gundersen K, Bergman B and Söderbäck E. 2006. Phosphorus-limited growth dynamics in two Baltic Sea cyanobacteria, Nodularia sp. and Aphanizomenon sp. FEMS Microbiology Ecology 58(3): 323–32. DOI: https://doi.org/10.1111/j.1574-6941.2006.00180.x

Hardy R, Burns R C and Holsten R D. 1973. Application of acetylene ethylene assay for measurement of nitrogen fixation. Soil Biology and Biochemistry 5(1): 47–81. DOI: https://doi.org/10.1016/0038-0717(73)90093-X

Haystead A, Robinson R and Stewart W D P. 1970. Nitrogenase activity in extracts of heterocystous and non-heterocystous blue-green algae. Arch. Mikrobiol. 74(3): 235–43. DOI: https://doi.org/10.1007/BF00408884

Hoyer M V, Frazer T K, Notestein S K and Canfield D E. 2002. Nutrient, chlorophyll, and water clarity relationships in Florida’s nearshore coastal waters with comparisons to freshwater lakes. Canadian Journal of Fisheries and Aquatic Sciences 59(6): 1024–31. DOI: https://doi.org/10.1139/f02-077

Khan M S, Zaidi A and Wani P A. 2007. Role of phosphate-solubilizing microorganisms in sustainable agriculture—a review. Agronomy for Sustainable Development 27(1): 29–43. DOI: https://doi.org/10.1051/agro:2006011

Mackinney G. 1941. Absorption of light by chlorophyll solutions. Journal of Biological Chemistry 140(2): 315–22. DOI: https://doi.org/10.1016/S0021-9258(18)51320-X

Meeuwig J J, Rasmussen J B and Peters R H.1998. Turbid waters and clarifying mussels: their moderation of empirical chknutrient relations in estuaries in Prince Edward Island, Canada. Marine Ecology Progress Series 171(1998): 139–50. DOI: https://doi.org/10.3354/meps171139

Natesan R and Shanmugasundaram S. 1989. Extracellular phosphate solubilization by the cyanobacterium Anabaena ARM 310. Journal of Bioscience 14: 203–8. DOI: https://doi.org/10.1007/BF02716680

Roychoudhury P and Kaushik B D. 1989. Solubilization of Mussorie rock phosphate by cyanobacteria. Current Science 58(10): 569–70.

Smith R C and Evans M C W. 1970. Soluble nitrogenase from vegetative cells of the blue-green alga Anabaena cylindrical. Nature 225(5239): 1253–4. DOI: https://doi.org/10.1038/2251253a0

Stanier R Y, Kunisawa R, Mandel M and Cohen-Bazier G. 1971. Purification and properties of unicellular blue green algae (Order Chroococcales). Bacteriological Reviews 35(2): 171–205. DOI: https://doi.org/10.1128/br.35.2.171-205.1971

Stewart W D P and Alexander G. 1971. Phosphorus availability and nitrogenase activity in aquatic blue-green algae. Freshwater Biology 1(4): 389–404. DOI: https://doi.org/10.1111/j.1365-2427.1971.tb01570.x

Venkataraman G S. 1961. The role of blue-green algae in agriculture. Science Culture 27: 9–13.

Watanabe F S and Olsen S R. 1965. Test of an ascorbic acid method for determining phosphorus in water and NaHCO3 extracts from soil. Soil Science Society of America Journal 29(6): 677–8. DOI: https://doi.org/10.2136/sssaj1965.03615995002900060025x

Whitton B A, Grainger S L J, Hawley G R W and Simon J W. 1991. Cell-bound and extracellular phosphatase activities of cyanobacterial isolates. Microbial Ecology 21(1): 85–98. DOI: https://doi.org/10.1007/BF02539146

Yandigeri M S and Pabbi S. 2005. Response of diazotrophic cyanobacteria to alternative sources of phosphorus. Indian Journal of Microbiology 45(2): 131.

Yandigeri M S, Yadav A K, Meena K K and Pabbi S. 2010. Effect of mineral phosphate on growth and nitrogen fixation of diazotrophic cyanobacteria Anabaena variabilis and Westiellopsis prolifica. Antonie van Leeuwenhoek 97(3): 297–306. DOI: https://doi.org/10.1007/s10482-009-9411-y

Yandigeri M S, Meena K K, Srinivasan R and Pabbi S. 2011. Effect of mineral phosphate solubilization on biological nitrogen fixation by diazotrophic cyanobacteria. Indian Journal of Microbiology 51(1): 48–53. DOI: https://doi.org/10.1007/s12088-011-0081-x

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2019-03-09

Published

2019-03-09

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How to Cite

JAISWAL, A., MISHRA, R., KOLI, D. K., SHARMA, V. K., & PABBI, S. (2019). Evaluation of growth, nitrogen fixation and P-solubilizing ability of diazotrophic cyanobacteria under mineral phosphorus sources. The Indian Journal of Agricultural Sciences, 89(3), 420–425. https://doi.org/10.56093/ijas.v89i3.87581
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