Gene identifieation of brown panthopper (Nilaparvata lugens) resistance in rice (Oryza sativa)

Abstract

A study was conducted during winter season of 2000-2001 to analyse the allelic relationship of brown planthopper (Nilapravata Lugens Stal) resistance gene (s) in 10 resistant cu1tivars with the 3 known genes. i.e Bph 3, bph 4 nad bph 6(t). The brown planthopper resistant cultivars 'Velluthacheera', 'T 1471', 'T 1426', 'T 1432', 'ARC 5984, 'Manoharsali', 'Sonasali' and 'Nagarjuna' were crossed with 'TN 1 ' (suspectible) and some of the known gene donors, viz 'Rathu Heenati' (Bph 3), 'IR 72' (Bph 6t), 'Babawee' (bph 4) while 'ARC 14529' and 'ARC 14771 ' were crossed with 'IR 72' only. All the parents, F1s and F2s were screened against brown planthopper population maintained at Hyderahad during 2000 arid 2001 under glasshouse conditions using standardized mass screening techniques. The segregation pattern in F2 generation of the crosses involving susceptible check 'TN 1' with 'Velluthacheera', 'T 1471'. 'T 1426' and 'T 1432' indicated 2 duplicate dominant genes, whereas crosses of 'ARC 5984', 'Manoharsali', 'Sonasali' and 'Nagarjuna' with 'TN 1 ' showed single recessive gene for resistance. The allelic test with known brown planthopper resistance genes suggested that 'Velluthacheera'. 'T 1471 ', 'T 1426', 'T 1432' possessed 2 dominant genes (Bph 3 and Bph 6t) while the genes present in 'ARC 14529' and 'ARC 14771' are allelic to that of 'IR 72' (Bph 6t) The other cultivars 'ARC 5984', 'Manoharsali', 'Sonasali' and 'Nagarjuna' possessed single recessive gene which is allelic to bph 4 present in 'Babawee'.