Integrated approach for management of root-knot nematode (Meloidogyne incognita) in bitter gourd (Momordica charantia)
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Keywords:
Bio-agents, Bitter gourd, Integrated Management, Meloidogyne incognita, Neem cakeAbstract
Field trials were conducted to manage root-knot nematode (Meloidogyne incognita) infesting bitter gourd (Momordica charantia L.). Four summer ploughings in May-June, 2014 at 15±2 days interval caused 67% reduction in M. incognita population. To develop an ecofriendly nematode management module organic amendment (neem cake, Azadirachta indica at 1.5 t/ha) and talc based preparations of bio-agents (Pseudomonas flourescens and Trichoderma harzianum) as seed treatment (10 g/kg) and soil application (10 kg/ha) were integrated with summer ploughings as pre-treatment. Carbofuran 3G at 1 kg a.i./ha was kept as control. The treatments were: T1- Control (Carbofuran); T2- Neem cake; T3- P. flourescens (seed treatment (10 g/kg seed as well as soil application at 10 kg/ha); T4- T. harzianum (seed treatment 10 g/kg seed as well as soil application at 10 kg/ha); T5- T2+T3+T4; T6-Farmers’ practice. The best protection of bitter gourd root-knot disease was achieved through integrated application of neem cake, P. flourencens and T. harzianum. Significantly better results (in terms of vine height and reduction in M. incognita population) were recorded under consequent second year trial (84 and 94% respectively) as compared to first year trial (47 and 73% respectively). It also enhanced marketable yield of bitter gourd significantly (P>0.5) (up to 108%) in the second season trial, as compared to farmers’ practice. It is suggested that integrated approach using organic amendment with bioagents to manage root-knot disease of bitter gourd under natural infestation is environmental friendly and beneï¬cial to growers/farmers’ having small holdings of land.Downloads
References
Anita B, Rajendaran G and Samiyappan R. 2004. Induction of systemic resistance in tomato against root-knot nematode, Meloidogyne incognita by Pseudomonas fluorescens. Nematologia Mediterranea 32: 47–51.
Anwar S A and McKenry M V. 2010. Incidence and reproduction of Meloidogyne incognita on vegetable crop genotypes. Pakistan Journal of Zoology 42: 135–41.
Asraf M S and Khan T A. 2010. Integrated approach for the management of Meloidogyne javanica on eggplant using oil cakes and bio-control agents. Archives of Phytopathology and Plant Protection 43:609–14. DOI: https://doi.org/10.1080/03235400801972434
Campbell P J H, Kloepper J, Jones J, Suslow T and Wilson M. 2006. Integrated biological control of bacterial speck and
spot of tomato under field conditions using foliar biological control and plant growth promoting rhizobacteria. Biological Control 358: 367–9.
Hussey R S. and Janssen G J W. 2002. Root-knot nematodes: Meloidogyne species. Plant Resistance to Parasitic Nematodes, pp 43-70. Starr J L, Cook R and Bridge J (Eds). CABI Publishing,Wallingford, UK. DOI: https://doi.org/10.1079/9780851994666.0043
Jain R K and Bhatti D S. 2008. Population development of rootknot nematode (Meloidogyne javanica) and tomato yield as influenced by summer ploughing. Tropical Pest Management 33: 122–4. DOI: https://doi.org/10.1080/09670878709371130
Jain R K, Mathur K N and Singh R V. 2007. Estimation of losses due to plant parasitic nematodes on different crops in India. Indian Journal of Nematology 37: 219–21.
Jegathambigai J, Wijeratnam R S W and Wijesundera R L C. 2011. Effect of Trichoderma viride and Trichoderma harzianumon Livistona rotundifolia root-knot nematode, Meloidogyne incognita. Journal of Entomology 8: 229–39. DOI: https://doi.org/10.3923/je.2011.229.239
Kaur S and Pathak M. 2011.Sources of resistance in Varh Karela (Momordica balsamina L) to root knot nematode. Plant Disease Research 26: 174.
Kloepper J W, Ryu C M and Zhang S. 2004. Induced systemic resistance and promotion of plant growth by Bacillus spp Phytopathology 1259: 1266–94. DOI: https://doi.org/10.1094/PHYTO.2004.94.11.1259
Poolperm S and Jiraunokoorskul W. 2017.An update review on the anthelmintic activity of bitter gourd, Momordica charantia. Pharmacognosy Reviews 11: 31–4. DOI: https://doi.org/10.4103/phrev.phrev_52_16
Sahebani N. and Hadavi N. 2008. Biological control of root-knot nematode, Meloidogyne javanica by Trichoderma harzianum. Journal of Soil Biology and Biochemistry 40: 2016–20. DOI: https://doi.org/10.1016/j.soilbio.2008.03.011
Singh S and Mathur N. 2010. In vitro studies of antagonistic fungi against the root-knot nematode, Meloidogyne incognita. Biocontrol Science and Technology 20: 275–85. DOI: https://doi.org/10.1080/09583150903484318
Singh S and Singh R K. 2012. Development of an integrated approach for managing root-knot disease on chili (Capsicum annum L) under field conditions. Russian Journal of Nematology 20: 65–73.
Singh S K, Conde B and Hodda M. 2012. Root-knot nematode (Meloidogyne incognita) on bitter melon (Momordica DOI: https://doi.org/10.1007/s13314-012-0052-z
charantia) near Darwin, Australia. Australasian Plant Disease Notes 7: 75–8.
Singh S. 2013. Integrated approach for the management of the root-knot nematode, Meloidogyne incognita, on eggplant under field conditions. Nematology 15: 747–57. DOI: https://doi.org/10.1163/15685411-00002715
Suarez B, Rey M and Castillo P. 2004. Isolation and characterization of PRA1, a trypsin-like protease from the biological control agent Trichoderma harzianum CECT 2413 displaying nematicidal activity. Applied Microbiology and Biotechnology 46: 55–65. DOI: https://doi.org/10.1007/s00253-004-1610-x
Timper P, Kone D, Yin J, Ji P, Gardner B B M. 2009. Evaluation of an antibiotic producing strain of Pseudomonas fluorescens for suppression of plant parasitic nematodes. Journal of Nematology 41: 234–40.
Verma A C, Singh H K and Khan M N. 2005. Management of rootknot nematode, Meloidogyne incognita, through antagonistic approaches in pointed gourd. Indian Journal of Nematology 35: 75–9.
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