Paclobutrazol induced physio-biochemical manifestations to ameliorate water deficit stress in rice (Oryza sativa)

NITIN KUMAR GARG; CHIRAG MAHESHWARI; SUSHIL S CHANGAN; VAIBHAV KUMAR; AMRESH KUMAR; ARCHANA SINGH; KISHWAR ALI; ARUNA TYAGI

doi:10.56093/ijas.v89i11.95305

Authors

NITIN KUMAR GARG Assistant Professor, Rajasthan Agricultural Research Institute, Durgapura, Jaipur;
CHIRAG MAHESHWARI Scientist, ICAR-CIAE, Bhopal
SUSHIL S CHANGAN Scientist, ICAR-CPRI, Shimla
VAIBHAV KUMAR Scientist, Basic Sciences Division, ICAR-IIPR, Kanpur
AMRESH KUMAR Research Scholar, ICAR-NRCPB, New Delhi
ARCHANA SINGH Principal Scientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
KISHWAR ALI Chief Technical officer, Division of Biochemistry, ICAR-IARI, New Delhi
ARUNA TYAGI Principal Scientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India

https://doi.org/10.56093/ijas.v89i11.95305

Keywords:

ABA, MSI, PBZ, PEG, Rice, RWC, Water deficit stress

Abstract

In this study, the mechanism of paclobutrazol (PBZ) mediated improvement in tolerance to water deficit in rice (Oryza sativa L.) genotypes were thoroughly investigated. Rice genotypes were subjected to different doses (0, 30, 60, 90, 120 ppm) of paclobutrazol. Study revealed that relative water content, membrane stability index, total chlorophyll and abscisic acid content significantly increased by application of PBZ in contrasting rice genotypes, i.e. Nagina-22 (drought-tolerant genotype), IR-64 (drought sensitive) and IR-64 DTY1.1 [drought-tolerant, near isogenic line of IR-64 developed by the introgression of a major QTL (qDTY1.1)] under polyethylene glycol mediated water deficit stress. The increase was found to be of higher magnitude in 90 ppm dose of paclobutrazol compared to other doses. Our results suggest that PBZ application could significantly improve tolerance in rice genotypes particularly susceptible genotype under limited water availability through selective changes in physio-biochemical parameters.

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