Phenotyping and marker based identification of resistant lines in wheat (Triticum aestivum) against spot blotch pathogen (Cochliobolus sativus)

DEVENDRA K CHOUDHARY; RASHMI AGGARWAL; BISHNU MAYA BASHYAL; V SHANMUGAM; JASDEEP C PADARIA

doi:10.56093/ijas.v89i11.95335

Authors

DEVENDRA K CHOUDHARY Ph D Scholar, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
RASHMI AGGARWAL Principal Scientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
BISHNU MAYA BASHYAL Senior Scientist, Division of Plant Pathology, ICAR-IARI
V SHANMUGAM Principal Scientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
JASDEEP C PADARIA Principal Scientist, National Institute for Plant Biotechnology, Pusa, New Delhi

https://doi.org/10.56093/ijas.v89i11.95335

Keywords:

PCR, Phenotyping, QTL, Spot blotch, SSR

Abstract

Spot blotch of wheat caused by Cochliobolus sativus is an emerging disease in hot and humid climate. Breeding for resistance is most suitable approach for management of this disease. A collection of 55 wheat (Triticum aestivum L.) genotypes were phenotyped for spot blotch resistance at seedling and adult plant stages. Based on phenotyping of 10 days old seedling Chirya 7, Chirya 3, Ning 8139, Suzhou, Milan-3, HD 2888, HD 2967, WR 95 were identified as resistant, while genotypes, viz. A-9-30-1, Agra local, C-306, K-68, Sonalika were highly susceptible. Phenotyping of adult plants stage revealed Chirya 7, Chirya 3, Ning 8139, Suzhou, Milan-3, HD 2888, HD 2967, WR 95, HW 3081 as resistant, whereas genotypes A-9-30-1, Agra local, C-306, Sonalika were highly susceptible. Phenotyping in adult and seedling plants showed a strong correlation of r = 0.91. DNA was isolated from all genotypes and was subjected to polymerase chain reaction (PCR) using simple sequence repeat (SSR) primers, viz. Xgwm148, Xgwm374 and Xgwm067, Xgwm371 for QTL Qsb.bhu-2B and Qsb.bhu-5B respectively. Presence and absence of different markers was confirmed by a unique amplification by PCR and visualized in Agarose gel electrophoresis. Two markers Xgwm371 and Xgwm374 showed significant association with resistance to B. sorokiniana and established to be linked with resistance since these were absent in highly susceptible genotypes. Hence, these markers could be useful in increasing the efficiency of selection for resistance to B. sorokiniana in wheat breeding and can be used in marker assisted selection.

Downloads

Download data is not yet available.

References

Adlakha K L, Wilcoxson R D and Raychaudhuri S P. 1984. Resistance of wheat to leaf spot caused by Bipolaris sorokiniana. Plant Disease 68: 320–1. DOI: https://doi.org/10.1094/PD-68-320

Gajjar K, Mishra G, Radhakrishnan T, Dodia S, Rathnakumar A, Kumar N and Kumar A. 2014. Validation of SSR markers linked to the rust and late leaf spot diseases resistance in diverse peanut genotypes. Australian Journal of Crop Science 8: 927–36.

Gurung S, Mamidi S, Bonman J M, Xiong M, Brown-Guedira G and Adhikari T B. 2014. Genome-wide association study reveals novel quantitative trait loci associated with resistance to multiple leaf spot diseases of spring wheat. PLoS One 9 (9): e108179. DOI: https://doi.org/10.1371/journal.pone.0108179

Kumar J, Schafer P, Huckelhoven R, Langen G, Baltruschat H, Stein E, Nagarajan S and Kogel K H. 2002. Bipolaris sorokiniana, a cereal pathogen of global concern: cytological and molecular approaches towards better control. Molecular Plant Pathology 3:185–95. DOI: https://doi.org/10.1046/j.1364-3703.2002.00120.x

Kumar U, Joshi A K, Kumar S, Chand R and Röder M S. 2009 Mapping of resistance to spot blotch disease caused by Bipolaris sorokiniana in spring wheat. Theoretical and Applied Genetics 118(4): 783–92. DOI: https://doi.org/10.1007/s00122-008-0938-5

Kumar U, Joshi A K, Kumar S, Chand R and Röder M S. 2010. Quantitative trait loci for resistance to spot blotch caused by Bipolaris sorokiniana in wheat (T. aestivum L) lines ‘Ning 8201’and ‘Chirya 3’. Molecular Breeding 26(3): 477–91. DOI: https://doi.org/10.1007/s11032-009-9388-2

Murray M G and Thompson W F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8(19): 4321–6. DOI: https://doi.org/10.1093/nar/8.19.4321

Nagarajan S and Kumar J. 1998. Foliar blights of wheat in India: germplasm improvement and future challenges for sustainable, high yielding wheat production, pp 52–8. Helminthosporium blights of Wheat: Spot Blotch and Tan spot. Duveiller E, Dubin H J, Reeves J and McNab A (Eds). Mexico, DF, mexico: CIMMYT.

Prasanna K, Aggarwal R, Banerjee R, Gupta S, Sharma S, Bashyal B M, Kumar J and Jindal M. 2013. Basis of resistance in wheat genotypes to Bipolaris sorokiniana causing spot blotch disease. Indian Phytopathology 66: 67–75.

Singh D P and Pankaj K. 2005. Method of scoring of leaf blight of wheat caused by Bipolaris sorokiniana (Sacc) Shoem on top two leaves at adult plant stage pp. 289–294. Integrated Plant Disease Management, pp 289-94. Sharma R C and Sharma J N) (Eds). Scientific Publishers, Jodhpur, India.

Tembo B, Sibiya J, Tongoona P and Tembo L. 2017. Validation of microsatellite molecular markers linked with resistance to Bipolaris sorokiniana in wheat (Triticum aestivum L). Journal of Agricultural Science 155: 1061–8. DOI: https://doi.org/10.1017/S0021859617000144

Young R A and Kelly J D. 1997. RAPD markers linked to three major anthracnose resistance genes in common bean. Crop Science 37(3): 940–6. DOI: https://doi.org/10.2135/cropsci1997.0011183X003700030039x

Zadoks J C, Chang T T and Konjak C F. 1974. A decimal code for the growth stages of cereals. Weed Research 14: 415–21. DOI: https://doi.org/10.1111/j.1365-3180.1974.tb01084.x