Characterization of bolting behaviour in garlic (Allium sativum) using SNP
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Authors
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YADWINDER KAUR
M Sc Student, Punjab Agricultural University, Ludhiana, Punjab 141 004, India
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R K DHALL
Associate Professor, Punjab Agricultural University, Ludhiana, Punjab 141 004, India
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PRITI SHARMA
Assistant Professor, School of Agricultural Biotechnology, Punjab Agricultural University,
Ludhiana, Punjab.
Keywords:
Garlic, Incomplete bolters, Non-bolters, SNPAbstract
The present research was conducted to evaluate the bolting (incomplete bolters and non-bolters) behaviour in garlic (Allium sativum L.) accessions collected from different geographical areas. A total of 96 genotypes collected from different sources were used for genotype sequencing to identify SNP but only 73 genotypes among them showed good amplification. A primer (AsLFY) was designed by using reference of already designed gene specific primer (gALFY-2) by Rotem et al. (2007). After amplification, sequencing of these 73 genotypes was done; SNP (CCGTCG) was at 301 bp from initiation codon, i.e. G for non-bolters genotypes and A for incomplete bolter genotypes. Thus, the identified SNP would help in detecting incomplete bolter and non-bolter genotypes at seedling stage which can be useful for garlic breeding programmes.Downloads
References
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