RILs development and its characterization for MLB resistance and flowering in maize (Zea mays)

BHUPENDER KUMAR; SHYAM BIR SINGH; VISHAL SINGH; KARAMBIR SINGH HOODA; PRAVIN KUMAR BAGARIA; KRISHAN KUMAR; HANS RAJ PRADHAN; SONU KUMAR; MEENAKSHI MEENAKSHI; PRADEEP BHATI; BRIJESH KUMAR; GARIMA CHATURVEDI; SUJAY RAKSHIT

doi:10.56093/ijas.v90i1.98677

Authors

BHUPENDER KUMAR Scientist, ICAR-Indian Institute of Maize Research, PAU Campus, Ludhiana 141 004, India
SHYAM BIR SINGH Principal Scientist, ICAR-Indian Institute of Maize Research, PAU Campus, Ludhiana 141 004, India
VISHAL SINGH Scientist, ICAR-Indian Institute of Maize Research, PAU Campus, Ludhiana 141 004, India
KARAMBIR SINGH HOODA Scientist, ICAR-Indian Institute of Maize Research, PAU Campus, Ludhiana 141 004, India
PRAVIN KUMAR BAGARIA Scientist, ICAR-Indian Institute of Maize Research, PAU Campus, Ludhiana 141 004, India
KRISHAN KUMAR Scientist, ICAR-Indian Institute of Maize Research, PAU Campus, Ludhiana 141 004, India
HANS RAJ PRADHAN SRF, ICAR-Indian Institute of Maize Research, PAU Campus, Ludhiana 141 004, India
SONU KUMAR SRF, ICAR-Indian Institute of Maize Research, PAU Campus, Ludhiana 141 004, India
MEENAKSHI MEENAKSHI SRF, ICAR-Indian Institute of Maize Research, PAU Campus, Ludhiana 141 004, India
PRADEEP BHATI SRF, ICAR-Indian Institute of Maize Research, PAU Campus, Ludhiana 141 004, India
BRIJESH KUMAR SRF, ICAR-Indian Institute of Maize Research, PAU Campus, Ludhiana 141 004, India
GARIMA CHATURVEDI SRF, ICAR-Indian Institute of Maize Research, PAU Campus, Ludhiana 141 004, India
SUJAY RAKSHIT Director, Indian Institute of Maize Research, PAU Campus, Ludhiana, India

https://doi.org/10.56093/ijas.v90i1.98677

Keywords:

Genetic study, Maydis Leaf Blight, Polygenic traits, Simple Sequence Repeats

Abstract

Maydis leaf blight (MLB) resistance and days to flowering are the important yield determining traits in maize. Breeding for MLB resistance and days to flowering can be accelerated by understanding their genetics and identifying genomic regions contributing for their expression. Two F₂s population with 338 and 349 individuals along with their recombinants inbred lines (RILs) having 283 and 277 individuals were developed from F₁ crosses HKIPC4P × CML269 and ESM113 × P72clXbrasil1117 for genetic studies of MLB resistance and flowering. The populations along with their parents were screened under artificially inoculated conditions at hot-spot sites during 2015–17. Race O inoculum was artificially inoculated in the leaf whorl of each plant at 4-6 leaf stage. The inoculation was repeated after 8-10 days of first inoculation to avoid any chance of disease escape. The partial dominance in F₁s, normal distribution patterns in F₂s and RILs for both the traits has indicated their polygenic nature. Correlation analysis found negative and significant association (P≤0.001) between disease scores and days to flowering across the populations. Total 250 simple sequence repeats (SSR) markers, uniformly selected from all linkage groups were used for parental polymorphism survey between parents of the populations contrasting for target traits. Of total 250 SSRs, 122 (48.8% polymorphism) were identified as polymorphic between either of the parents. Sufficient genetic variation was observed within and between different F₂s and RILs mapping populations. The information on inheritance, parental polymorphism survey and genetic materials developed will be useful for fine mapping and systematic breeding of targeted traits in tropical maize germplasm.

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