Improvement of salinity stress in cumin (Cuminum cyminum) seedling by inoculation with Rhizobacteria

RAMIN PIRI; ALI MORADI; HAMIDREZA BALOUCHI

doi:10.56093/ijas.v90i2.99025

Authors

RAMIN PIRI MSc Scholar, Yasouj University, Daneshjoo St, Yasouj, Iran
ALI MORADI Associate Professor, Yasouj University, Daneshjoo St, Yasouj, Iran
HAMIDREZA BALOUCHI Associate Professor (Corresponding author), Department of Agronomy and Plant Breeding, Faculty of Agriculture, Yasouj University, Yasouj, Iran

https://doi.org/10.56093/ijas.v90i2.99025

Keywords:

Bacillus subtilis, Medicinal plant, Osmotic stress, Pseudomonas fluorescens, Seed germination

Abstract

Cumin (Cuminum cyminum L.) is the second most popular spice in the world, after black pepper, which is sensitive to salinity. In order to investigate the effect of seed bio-inoculation with plant growth promoting Rhizobacteria on germination and seedling indices of cumin under salinity stress, an experiment was laid out based on a completely randomized design with two factors and four replications in 2017 at Seed Science and Technology Laboratory, Yasouj University, Iran. Experimental factors included bio-inoculation with 3 strains, viz. PF2, PF25, and CHA0 of Pseudomonas fluorescents, and Bacillus subtilis and non-primed (control) and three levels of salinity stress (0, -4 and -8 bar). Results indicated that salt stress reduced germination (up to 40%) and seedling indices of cumin under -4 and -8 bar, the highest germination percentage, germination rate, seedling length, and seedling vigor index were achieved in the seeds inoculated with P. fluorescens, CHA0. At all levels of stress, seed bio-inoculation were increasing the seed soluble protein content compared to non-primed. The effect of this treatment was more obvious under salinity potential of -8 bar. The results indicated that salinity can affect cumin seed germination and the beneficial effect of PGPR could be used for improving its salt tolerance.

Downloads

Download data is not yet available.

References

Abdul-Baki A A and Anderson J D. 1970. Viability and leaching of sugars from germinating barley. Crop Science 10: 31–4. DOI: https://doi.org/10.2135/cropsci1970.0011183X001000010012x

Amini Dehaghi M and Mollafilabi A. 2011. Evaluation of some drought resistance criteria in landraces. American-Eurasian network for scientific information. Advances in Environmental Biology 5(2): 237–42.

Bradford M. 1976. A rapid and sensitive method for the quantitation of protein utilizing the principle of protein dye binding. Annual Review of Biochemistry 72: 248–54. DOI: https://doi.org/10.1016/0003-2697(76)90527-3

Datta J K, Nag S, Banerjee A and Mondal N K. 2009. Impact of salt stress on five varieties of wheat (Triticum aestivum L.) cultivars under laboratory condition. Journal of Applied Sciences and Environmental Management 13: 93–7. DOI: https://doi.org/10.4314/jasem.v13i3.55372

De R and Kar R K. 1994. Seed germination and seedling growth of mung bean (Vigna radiata) under water stress induced by PEG-6000. Seed Science and Technology 23: 301–8.

Demidchik Grosskinsky K, Tafner R, Moreno V M, Stenglein S A, García de Salamone I E, Nelson L M, Novak O, Strnad M, Graaff E V and Roitsch Th. 2016. Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis. Scientific Reports 6: 1–11. DOI: https://doi.org/10.1038/srep23310

Ellis R H and Roberts E H. 1981. The quantification of ageing and survival in orthodox seeds. Seed Science and Technology 9: 373–409.

Gagandeep Dhanalakshmi S, Méndiz E, Rao A R and Kale R K. 2003. Chemopreventive effects of Cuminum cyminum in chemically induced forestomach and uterine cervix tumors in murine model systems. Nutrition and Cancer 47: 171–80 DOI: https://doi.org/10.1207/s15327914nc4702_10

Ghezal N, Rinez I, Sbai H, Saad I, Farooq M, Rinez A, Zribi I and Haouala R. 2016. Improvement of Pisum sativum salt stress tolerance by bio-priming their seeds using Typha angustifolia leaves aqueous extract. South African Journal of Botany 105: 240–50. DOI: https://doi.org/10.1016/j.sajb.2016.04.006

Habib Sh H, Hossain K and Halimi M S. 2016. plant growthpromoting rhizobacteria enhance salinity stress tolerance in okra through ROS-scavenging enzymes. BioMed Research International 10: 1–10. DOI: https://doi.org/10.1155/2016/6284547

Hassanzadeh delouei M, Vazin F and Nadaf J. 2013. Effect of salt stress in different stages of growth on qualitative and quantitative characteristics of cumin (Cuminum cyminum L.). Agronomical Research in Moldavia 45(1): 89–97. DOI: https://doi.org/10.2478/v10298-012-0078-6

Ibrahim H I M. 2016. Tolerance of two pomegranates cultivars (Punica granatum L.) to salinity stress under hydroponic culture conditions. Journal of Applied Sciences Research 6(4): 38–46.

ISTA 2010. International Rules for Seed Testing. International Seed Testing Association, Bassersdorf, Switzerland.115pp.

Kaya M D, Ipek A and Ozturk A. 2003. Effects of different soil salinity levels on germination and seedling growth of safflower (Carthamus tinctorius L.). Turkish Journal of Agriculture and Forestry 27: 221–7.

Kaymak H A, Guvenc I, Yarali F and Denmez M F. 2009. The effects of bio-priming with PGPR on germination of radish (Raphanus sativus L.) seeds under saline conditions. Turkish Journal of Agriculture and Forestry 33: 173–9. DOI: https://doi.org/10.3906/tar-0806-30

Keshavarzi M H B. 2011. Effect of salt stress on germination and early seedling growth of savory (Satureja hortensis). Australian Journal of Basic and Applied Sciences 5(2): 3274–9.

Metwalil E M R, Abdelmoneim S T S, Bakheit M A and Kadasa N M S. 2015. Alleviation of salinity stress in faba bean (Vicia faba L.) plants by inoculation with plant growth promoting rhizobacteria (PGPR). Plant Omics Journal 8(5): 449–60.

Mohammadizad H A, Mirzakhani Gh, Ghafari M, Samavatipour P, Araghi S M and Fatehi M F. 2014. Effect of NaCl stress on seed germination indices and early seedling growth of cumin (Cuminum cyminum L.). Agriculture Science Developments 3(2): 161–6.

Neamatollahi E, Bannayan M, Souhani Darban A and Ghanbari A. 2009. Hydropriming and osmopriming effects on cumin (Cuminum cyminum L.) seeds germination. International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering 3(9): 477–80.

Piri R, Moradi A, Salehi A and Balouchi H. 2016. The effect of seed bio-priming with Pseudomonas fluorescents on germination and seedling indices of cumin (Cuminum cyminum L.) under drought stress. (In) The Fifth National Congress on Medicinal Plants. Isfahan (Iran), May 18–19.

Roodbari N, Lahooti M, Aein A, ganjali A and Roodbari Sh. 2013. The effect of salinity stress on germination and seedling growth of cumin (Cuminum cyminum L.). Journal of Agriculture and Food Technology 3(5): 1–4.

Shoor M, Afrousheh M, Rabeie J and Vahidi M, 2014. The effect of salinity priming on germination and growth stage of Cumin (Cuminum cyminum L.). Research Journal of Agriculture and Environmental Management 3(7): 340–52.

Srivastava L M. 2003. Plant Growth and Development. Hormones and the Environment. Oxford: Academic Press. The Annals of Botany Company 722pp.

Van’t Hoff J H. 1887. The role of osmotic pressure in the analogy between solution and gases. Zeitschrift für Physikalische Chemie 1: 481–508. DOI: https://doi.org/10.1515/zpch-1887-0151

Weller D M and Cook R J. 1983. Suppression of take-all of wheat by seed treatments with Pseudomonads fluorescent. Journal of Phytopathology 78: 463–9. DOI: https://doi.org/10.1094/Phyto-73-463