Extraction of phlorotannin-rich polyphenolic compounds from brown macroalgae: Promising antioxidants for functional foods

Kajal Chakraborty; Ans Mariya  George; Ashley  Shaijo; Leena Devadas  Marypushpam; Shubhajit  Dhara; Silpa Kunnapilly Paulose

doi:10.21077/

Authors

Kajal Chakraborty Central Marine Fisheries Research Institute
Ans Mariya George 1Central Marine Fisheries Research Institute, Marine Biotechnology Fish Nutrition and Health Division Cochin, Ernakulam North P.O., P.B. No. 1603, Kerala, India 2Faculty of Marine Sciences, Lakeside Campus, Cochin University of Science and Technology, Cochin, Kerala State, India
Ashley Shaijo Marine Biotechnology Fish Nutrition and Health Division , Central Marine Fisheries Research Institute
Leena Devadas Marypushpam Marine Biotechnology Fish Nutrition and Health Division , Central Marine Fisheries Research Institute
Shubhajit Dhara Marine Biotechnology Fish Nutrition and Health Division , Central Marine Fisheries Research Institute
Silpa Kunnapilly Paulose Marine Biotechnology Fish Nutrition and Health Division , Central Marine Fisheries Research Institute

https://doi.org/10.21077/

Keywords:

Antioxidants; Oxidative stress; Phlorotannin-rich polyphenolic compounds; Macroalgae

Abstract

Highly polymerised phlorotannin-based polyphenolic compounds are ubiquitous yet unique in brown macroalgae. They are potent antioxidants owing to the presence of pluralities of hydroxyl groups enabling them to scavenge numerous free radicals simultaneously and protect cells from oxidative damage. The present study aimed for a comparison of two solvent systems; ethanol:water:acetic acid (E:W:A, 70:29.7:0.3, v/v/v) and acetone:water:acetic acid (A:W:A, 70:29.7:0.3, v/v/v) for polyphenol extraction. The polyphenol extracts of seven brown algae of Sargassaceae family (Turbinaria ornata, T. conoides, T. decurrens, Sargassum ilicifolium, S. tenerrimum, S. wightii and S. plagiophyllum) were analysed for the total phenolic contents (TPC) (76.76-143.56 mg GAE g-1), total phloroglucinol contents (TPhC) (5.0119.77 mg PGE g-1) and antioxidant activities (IC₅₀ < 0.6 mg ml-1). S. plagiophyllum displayed the highest phloroglucinol content (19.77 mg PGE g-1) followed by T. ornata (13.26 mg PGE g-1) and T. decurrens (11.68 mg PGE g-1). S. plagiophyllum exhibited the strongest antioxidant activity, with the lowest IC₅₀ values in both the DPPH (0.29 mg ml-1) and ABTS (0.09 mg ml-1) assays. These results demonstrate the efficiency of the ethanol solvent system in producing a highly antioxidative, phlorotannin-rich polyphenolic concentrate from macroalgal extracts.

Keywords: Hydroxyl groups, Oxidative stress, Phloroglucinol, Total phenolic contents

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Author Biographies

Kajal Chakraborty, Central Marine Fisheries Research Institute

Senior Scientist, Marine Biotechnology Division
Ans Mariya George, 1Central Marine Fisheries Research Institute, Marine Biotechnology Fish Nutrition and Health Division Cochin, Ernakulam North P.O., P.B. No. 1603, Kerala, India 2Faculty of Marine Sciences, Lakeside Campus, Cochin University of Science and Technology, Cochin, Kerala State, India

JRF, Central Marine Fisheries Research Institute
Ashley Shaijo, Marine Biotechnology Fish Nutrition and Health Division , Central Marine Fisheries Research Institute

Research Scholar, Central Marine Fisheries Research Institute
Leena Devadas Marypushpam, Marine Biotechnology Fish Nutrition and Health Division , Central Marine Fisheries Research Institute

Research Scholar, Central Marine Fisheries Research Institute
Shubhajit Dhara, Marine Biotechnology Fish Nutrition and Health Division , Central Marine Fisheries Research Institute

Research Scholar, Central Marine Fisheries Research Institute
Silpa Kunnapilly Paulose, Marine Biotechnology Fish Nutrition and Health Division , Central Marine Fisheries Research Institute

Research Scholar, Central Marine Fisheries Research Institute

References

Aminina, N. M., Karaulova, E. P., Vishnevskaya, T. I., Yakush, E. V., Kim, Y. K., Nam, K. H., & Son, K. T. (2020). Characteristics of Polyphenolic Content in Brown Algae of the Pacific Coast of Russia. Molecules (Basel, Switzerland), 25(17), 3909. https://doi.org/10.3390/molecules25173909

Begum, R., Howlader, S., Mamun-Or-Rashid, A. N. M., Rafiquzzaman, S. M., Ashraf, G. M., Albadrani, G. M., Sayed, A. A., Peluso, I., Abdel-Daim, M. M., & Uddin, M. S. (2021). Antioxidant and Signal-Modulating Effects of Brown Seaweed-Derived Compounds against Oxidative Stress-Associated Pathology. Oxidative medicine and cellular longevity, 2021, 9974890. https://doi.org/10.1155/2021/9974890

Catarino MD, Silva AMS, Mateus N, Cardoso SM. Optimization of Phlorotannins Extraction from Fucus vesiculosus and Evaluation of Their Potential to Prevent Metabolic Disorders. Marine Drugs. 2019; 17(3):162. https://doi.org/10.3390/md17030162

Cory, H., Passarelli, S., Szeto, J., Tamez, M., & Mattei, J. (2018). The Role of Polyphenols in Human Health and Food Systems: A Mini-Review. Frontiers in nutrition, 5, 87. https://doi.org/10.3389/fnut.2018.00087

Cotas, J., Leandro, A., Monteiro, P., Pacheco, D., Figueirinha, A., Gonçalves, A. M. M., da Silva, G. J., & Pereira, L. (2020). Seaweed Phenolics: From Extraction to Applications. Marine drugs, 18(8), 384. https://doi.org/10.3390/md18080384

El-Beltagi, H. S., Mohamed, A. A., Mohamed, H. I., Ramadan, K. M. A., Barqawi, A. A., & Mansour, A. T. (2022). Phytochemical and Potential Properties of Macroalgae and Their Recent Applications: A Review. Marine drugs, 20(6), 342. https://doi.org/10.3390/md20060342

Farasat, M., Khavari-Nejad, R. A., Nabavi, S. M., & Namjooyan, F. (2014). Antioxidant Activity, Total Phenolics and Flavonoid Contents of some Edible Green Macroalgae from Northern Coasts of the Persian Gulf. Iranian journal of pharmaceutical research :IJPR, 13(1), 163–170

Generalić Mekinić I., Skroza D., Šimat V., Hamed I., Čagalj M., Popović Perković Z. (2019). Phenolic content of brown algae (pheophyceae) species: Extraction, identification, and quantification. Biomolecules. ;9:244. doi: 10.3390/biom9060244.

Farasat, M., Khavari-Nejad, R. A., Nabavi, S. M., & Namjooyan, F. (2014). Antioxidant Activity, Total Phenolics and Flavonoid Contents of some Edible Green Seaweeds from Northern Coasts of the Persian Gulf. Iranian journal of pharmaceutical research : IJPR, 13(1), 163–170.

Hatami, T., Emami, S. A., Miraghaee, S. S., & Mojarrab, M. (2014). Total Phenolic Contents and Antioxidant Activities of Different Extracts and Fractions from the Aerial Parts of Artemisia biennis Willd. Iranian journal of pharmaceutical research : IJPR, 13(2), 551–559

Heffernan N., Brunton N.P., FitzGerald R.J., Smyth T.J. Profiling of the molecular weight and structural isomer abundance of macroalgae-derived phlorotannins. Marine drugs. 2015;13:509–528. doi: 10.3390/md13010509.

Ho Lee, B., Jeong Hwang, H., Lomartire, S., & M Gonçalves, A. M. (2023). Marine Macroalgae Polyphenols as Potential Neuroprotective Antioxidants in Neurodegenerative Diseases. Marine drugs, 21(5), 261. https://doi.org/10.3390/md21050261

Kumar, Y., Tarafdar, A., Kumar, D., Saravanan, C., Badgujar, P. C., Pharande, A., Pareek, S., & Fawole, O. A. (2022). Polyphenols of Edible Macroalgae: Estimation of In Vitro Bio-Accessibility and Cytotoxicity, Quantification by LC-MS/MS and Potential Utilization as an Antimicrobial and Functional Food Ingredient. Antioxidants (Basel, Switzerland), 11(5), 993. https://doi.org/10.3390/antiox11050993

Li, Y., Fu, X., Duan, D., Liu, X., Xu, J., & Gao, X. (2017). Extraction and Identification of Phlorotannins from the Brown Alga, Sargassum fusiforme (Harvey) Setchell. Marine drugs, 15(2), 49. https://doi.org/10.3390/md15020049

Maheswari, V., & Babu, P. A. S. (2022). Phlorotannin and its Derivatives, a Potential Antiviral Molecule from Brown Macroalgae, an Overview. Russian journal of marine biology, 48(5), 309–324. https://doi.org/10.1134/S1063074022050169

Mathew, S., Abraham, T. E., & Zakaria, Z. A. (2015). Reactivity of phenolic compounds towards free radicals under in vitro conditions. Journal of food science and technology, 52(9), 5790–5798. https://doi.org/10.1007/s13197-014-1704-0

Menaa, F., Wijesinghe, U., Thiripuranathar, G., Althobaiti, N. A., Albalawi, A. E., Khan, B. A., & Menaa, B. (2021). Marine Algae-Derived Bioactive Compounds: A New Wave of Nanodrugs? Marine drugs, 19(9), 484. https://doi.org/10.3390/md19090484

Pereira, L., & Cotas, J. (2023). Therapeutic Potential of Polyphenols and Other Micronutrients of Marine Origin. Marine drugs, 21(6), 323. https://doi.org/10.3390/md21060323

Sánchez-Camargo, A. D. P., Montero, L., Stiger-Pouvreau, V., Tanniou, A., Cifuentes, A., Herrero, M., & Ibáñez, E. (2016). Considerations on the Use of Enzyme-Assisted Extraction in Combination with Pressurized Liquids to Recover Bioactive Compounds from Algae. Food chemistry, 192, 67–74. https://doi.org/10.1016/J.foodchem.2015.06.098

Santos, S. A. O., Félix, R., Pais, A. C. S., Rocha, S. M., & Silvestre, A. J. D. (2019). The Quest for Phenolic Compounds from Macroalgae: A Review of Extraction and Identification Methodologies. Biomolecules, 9(12), 847. https://doi.org/10.3390/biom9120847

Seo, C., Jeong, S. J., Yun, H. J., Lee, H. J., Lee, J. W., An, H. W., Han, N., Jung, W. K., & Lee, S. G. (2022). Nutraceutical Potential of Polyphenol-rich Sargassum Species Grown off the Korean coast: a Review. Food science and biotechnology, 31(8), 971–984. https://doi.org/10.1007/s10068-022-01050-x

Thambi, A., & Chakraborty, K. (2022). Brown and Red Marine Macroalgae as Novel Bioresources of Promising Medicinal Properties. Journal of Aquatic Food Product Technology, 31(3), 227–241. https://doi.org/10.1080/10498850.2022.2035877

Tziveleka, L. A., Tammam, M. A., Tzakou, O., Roussis, V., & Ioannou, E. (2021). Metabolites with Antioxidant Activity from Marine Macroalgae. Antioxidants (Basel, Switzerland), 10(9), 1431. https://doi.org/10.3390/antiox10091431

Wojdylo, A., Oszmianski, J. & Czemerys, R. (2007). Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chemistry, 105, 940-949.

Zhan, X., Chen, Z., Chen, R., & Shen, C. (2022). Environmental and Genetic Factors Involved in Plant Protection-Associated Secondary Metabolite Biosynthesis Pathways. Frontiers in plant science, 13. https://doi.org/10.3389/fpls.2022.877304

Zheng, H., Zhao, Y., & Guo, L. (2022). A Bioactive Substance Derived from Brown Macroalgae: Phlorotannins. Marine drugs, 20(12), 742. https://doi.org/10.3390/md20120742