Evaluation of human chorionic gonadotropin (HCG) dose and latency period combinations on the weight of stripped eggs during induced spawning of Clarias batrachus (Linn.)

Abstract

The weight of stripped egg was evaluated as breeding performance of Clarias batrachus, administered with five doses of human chorionic gonadotropin (HCG) (1000, 2000, 3000, 4000 and 5000 IU kg-1 body weight of female) in combination with five latency periods (11, 14, 17, 20 and 23 h). The females did not respond to stripping at 1000-3000 IU dose in combination with 11 h latency. The weight of stripped eggs significantly (p<0.05) increased while prolonging the latency period to 20 or 23 h at 1000 IU dose compared to other latencies. The females were stripped and free flow of eggs was observed at 3000 and 4000 IU dose with 14-23 h latency combinations. The stripped egg weight at those combinations was significantly (p<0.05) higher compared to other dose and latency combinations. It was also observed that lower weight of eggs could be collected at 5000 IU dose in combination with 17-23 h post-injection. So it is wise to strip C. batrachus female while injecting 3000-4000 IU kg-1 body weight and latency period of 14-23 h to get highest weight of stripped eggs.