Diagnostic potentiality of fractionated antigenof Aeromonas hydrophila isolated from gold fish,Carassius auratus (Linn.)


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Authors

  • S Maji West Bengal University of Animal and Fishery Sciences,Mohanpur Campus, P.O. - B.C.K.V., Pin. - 741252, West Bengal, India
  • P Mali West Bengal University of Animal and Fishery Sciences,Mohanpur Campus, P.O. - B.C.K.V., Pin. - 741252, West Bengal, India
  • S.N. Joardar West Bengal University of Animal and Fishery Sciences,Mohanpur Campus, P.O. - B.C.K.V., Pin. - 741252, West Bengal, India

Abstract

With the aim of isolating purified antigens of extra cellular products (ECP) ofA. hydrophila that might be exploited for early sero-diagnosis, bacteria wasisolated from ulcerative lesions of gold fish. Polypeptide profile of ECP of A.hydrophila showed 12 bands having molecular weight in the range of 22 – 55kDa in SDS-PAGE analysis. Fractionation (By DEAE-cellulose) and serologi-cal characterization of ECP were subsequently performed. One of thefractionated antigen (D1) possessing mainly of 45 kDa polypeptide revealedsero-reactivity with A. hydrophila antiserum having no cross-reactivecomponent(s) with related bacterial antigens, viz. Vibrio harveyi, V.alginolyticus and Pseudomonas fluorescence. Hence, 45 kDa polypeptide of ECPof A. hydrophila showing potentiality of sero-diagnosis should be given dueattention.

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Author Biographies

  • S Maji, West Bengal University of Animal and Fishery Sciences,Mohanpur Campus, P.O. - B.C.K.V., Pin. - 741252, West Bengal, India
    West Bengal University of Animal and Fishery Sciences,Mohanpur Campus, P.O. - B.C.K.V., Pin. - 741252, West Bengal, India
  • P Mali, West Bengal University of Animal and Fishery Sciences,Mohanpur Campus, P.O. - B.C.K.V., Pin. - 741252, West Bengal, India
    West Bengal University of Animal and Fishery Sciences,Mohanpur Campus, P.O. - B.C.K.V., Pin. - 741252, West Bengal, India
  • S.N. Joardar, West Bengal University of Animal and Fishery Sciences,Mohanpur Campus, P.O. - B.C.K.V., Pin. - 741252, West Bengal, India
    West Bengal University of Animal and Fishery Sciences,Mohanpur Campus, P.O. - B.C.K.V., Pin. - 741252, West Bengal, India

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Issue

Vol. 53 No. 1 (2006)

Section

Articles

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The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.

How to Cite

Maji, S., Mali, P., & Joardar, S. (2011). Diagnostic potentiality of fractionated antigenof Aeromonas hydrophila isolated from gold fish,Carassius auratus (Linn.). Indian Journal of Fisheries, 53(1), 13-18. https://epubs.icar.org.in/index.php/IJF/article/view/6888