Use of comet assay for genotoxicity assessment in fishes from Gomti River


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Authors

  • N S Nagpure National Bureau of Fish Genetics Resources, Canal Ring Road, Telibagh, P.O. Dilkusha Lucknow - 226 002, India
  • Shilpi Sharma National Bureau of Fish Genetics Resources, Canal Ring Road, Telibagh, P.O. Dilkusha Lucknow - 226 002, India
  • Sanjay Pandey National Bureau of Fish Genetics Resources, Canal Ring Road, Telibagh, P.O. Dilkusha Lucknow - 226 002, India
  • Ravindra Kumar National Bureau of Fish Genetics Resources, Canal Ring Road, Telibagh, P.O. Dilkusha Lucknow - 226 002, India
  • Satish K Srivastava National Bureau of Fish Genetics Resources, Canal Ring Road, Telibagh, P.O. Dilkusha Lucknow - 226 002, India
  • M S Verma National Bureau of Fish Genetics Resources, Canal Ring Road, Telibagh, P.O. Dilkusha Lucknow - 226 002, India
  • D Kapoor National Bureau of Fish Genetics Resources, Canal Ring Road, Telibagh, P.O. Dilkusha Lucknow - 226 002, India

Abstract

An increasing number of genotoxic chemicals are being released into the aquatic environment, posing serious damage to our

rich aquatic biodiversity and also indirectly to human health. In view of the above, attempts have been made to explore the

feasibility of application of comet assay for biomonitoring of fish from polluted sites of River Gomti in Lucknow (Uttar

Pradesh), which has been exposed to heavy pollution during the recent years. For comet assay, blood samples of two fish

species found in the river namely

Labeo rohita and Puntius puntius

, were tested. Blood samples from fishes of the same

species collected from the fish ponds of the National Bureau of Fish Genetic Resources (NBFGR), Lucknow were taken as

control for comparison. The samples were then subjected to comet assay for assessing DNA damage. Significant differences

were observed with respect to the two important comet parameters

viz.

, percentage tail DNA and tail length as compared to

control. The results indicated the sensitivity and suitability of comet assay for '

in situ

' biomonitoring programmes to explore

genotoxicity of environmental pollutants.

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Issue

Vol. 55 No. 3 (2008)

Section

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The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.

How to Cite

Nagpure, N. S., Sharma, S., Pandey, S., Kumar, R., Srivastava, S. K., Verma, M. S., & Kapoor, D. (2011). Use of comet assay for genotoxicity assessment in fishes from Gomti River. Indian Journal of Fisheries, 55(3), 285-288. https://epubs.icar.org.in/index.php/IJF/article/view/6904