Induction of mx gene expression in the Indian major carp, rohu (Labeo rohita, Linnaeus)


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Authors

  • Parvati Sharma Department of Zoology and Aquaculture, CCS Haryana Agricultural University Hisar - 125 004, India
  • S K Gahlawat Department of Zoology and Aquaculture, CCS Haryana Agricultural University Hisar - 125 004, India
  • R C Sihag National Research Centre on Equines, Hisar, India
  • Mamta Chauhan National Research Centre on Equines, Hisar, India

Abstract

Mx genes are inducible by type I interferon and involved in antiviral defenses in vertebrates. In the present investigation, the protocol for obtaining Mx gene expression was standardized by
Poly I:C injection in Labeo rohita. A 786 bp cDNA was obtained on day 3 after poly I:C injection. PCR amplification of this cDNA using Mx specific primers also yielded 786 bp product. However,
PBS injected fishes did not show any Mx expression.

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Author Biographies

  • Parvati Sharma, Department of Zoology and Aquaculture, CCS Haryana Agricultural University Hisar - 125 004, India
    Department of Zoology and Aquaculture, CCS Haryana Agricultural University
    Hisar - 125 004, India
  • S K Gahlawat, Department of Zoology and Aquaculture, CCS Haryana Agricultural University Hisar - 125 004, India
    Department of Zoology and Aquaculture, CCS Haryana Agricultural University
    Hisar - 125 004, India
  • R C Sihag, National Research Centre on Equines, Hisar, India
    National Research Centre on Equines, Hisar, India
  • Mamta Chauhan, National Research Centre on Equines, Hisar, India
    National Research Centre on Equines, Hisar, India

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Issue

Vol. 54 No. 3 (2007)

Section

Articles

License

The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.

How to Cite

Sharma, P., Gahlawat, S. K., Sihag, R. C., & Chauhan, M. (2011). Induction of mx gene expression in the Indian major carp, rohu (Labeo rohita, Linnaeus). Indian Journal of Fisheries, 54(3), 321-326. https://epubs.icar.org.in/index.php/IJF/article/view/6948