Randomly amplified polymorphic DNA (RAPD)fingerprinting of Schizothorax richardsonii (Gray)


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Authors

  • Rajeev Kapila National Research Centre on Coldwater Fisheries, Bhimtal –263136 (Nainital) UA
  • D P Mishra Department of Biochemistry, G.B. Pant University of Agriculture and Technology.,Pantnagar- 263145 UA

Abstract

Randomly amplified polymorphic DNA (RAPD) fingerprinting was used to assessthe genetic variation/similarity among snow trout (Schizothorax richardsonii)collected from four wild locations. RAPD profiles were generated using tenoligonucleotide pimers which produced repeatable and consistent results. Ninetyeight RAPD loci were detected with average of 9.8 bands per primer. Sixty nineof these loci were  polymorphic exhibiting location specific as well as individualspecific bands. Twenty nine of the loci exhibited species specific monomorphism.Dendrograms prepared using unweighted pair group method with arithmeticaverages (UPGMA) using Jaccard’s similarity coefficient revealed fishes fromneighboring locations were more genetically similar than the distant ones.

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Author Biographies

  • Rajeev Kapila, National Research Centre on Coldwater Fisheries, Bhimtal –263136 (Nainital) UA
    National Research Centre on Coldwater Fisheries, Bhimtal –263136 (Nainital) UA
  • D P Mishra, Department of Biochemistry, G.B. Pant University of Agriculture and Technology.,Pantnagar- 263145 UA
    Department of Biochemistry,  G.B. Pant University of Agriculture and Technology.,Pantnagar- 263145 UA

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Issue

Vol. 53 No. 2 (2006)

Section

Note

License

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How to Cite

Kapila, R., & Mishra, D. P. (2011). Randomly amplified polymorphic DNA (RAPD)fingerprinting of Schizothorax richardsonii (Gray). Indian Journal of Fisheries, 53(2), 219-224. https://epubs.icar.org.in/index.php/IJF/article/view/6983