Development of cell culture from liver and kidney of Indian major carp, Labeo rohita (Hamilton)


248 / 299

Authors

  • W S Lakra Division of Fish Genetics and Biotechnology, Central Institute of Fisheries Education, Versova, Mumbai 400 061
  • M R Behera Division of Fish Genetics and Biotechnology, Central Institute of Fisheries Education, Versova, Mumbai 400 061
  • N Sivakumar Division of Fish Genetics and Biotechnology, Central Institute of Fisheries Education, Versova, Mumbai 400 061
  • M Goswami Division of Fish Genetics and Biotechnology, Central Institute of Fisheries Education, Versova, Mumbai 400 061
  • R R Bhonde National Center for Cell Science, Ganeshkhind, Pune 411 007.

Abstract

The present study was undertaken to assess the potential of six tissues of
Labeo rohita viz. kidney, liver, heart, gill, caudal fin and swim bladder for
attachment, cell outgrowth and proliferation by explant culture and
trypusinization methods. Cell outgrowth was obtained from liver explants of
L. rohita (2 months old) on ninth day. Cells radiated from liver explants grew
and gave rise to colonies of individual cells. The disaggregated cells prepared
by trypsinization of kidney tissue reached confluent stage nine days after
seeding. The optimum incubation temperature for the liver and kidney cells
was found to be 26-28ºC. Among the various media [Leibovitz-15 (L-15), M-
199 and EMEM] tested, L-15 was found to be most suitable for the attachment
and cell proliferation. The media were supplemented with 15 % foetal bovine
serum, 10 % fish muscle extract (FME) and 1 % fish (L. rohita) serum. Different
pH of the growth media ranging from 7.2 to 7.8 was employed to determine
optimum values. It was found that pH 7.4 proved to be the best for growth of
cells.

Downloads

Download data is not yet available.

Author Biographies

  • W S Lakra, Division of Fish Genetics and Biotechnology, Central Institute of Fisheries Education, Versova, Mumbai 400 061
    Division of Fish Genetics and Biotechnology, Central Institute of Fisheries Education,
    Versova, Mumbai 400 061
  • M R Behera, Division of Fish Genetics and Biotechnology, Central Institute of Fisheries Education, Versova, Mumbai 400 061
    Division of Fish Genetics and Biotechnology, Central Institute of Fisheries Education,
    Versova, Mumbai 400 061
  • N Sivakumar, Division of Fish Genetics and Biotechnology, Central Institute of Fisheries Education, Versova, Mumbai 400 061
    Division of Fish Genetics and Biotechnology, Central Institute of Fisheries Education,
    Versova, Mumbai 400 061
  • M Goswami, Division of Fish Genetics and Biotechnology, Central Institute of Fisheries Education, Versova, Mumbai 400 061
    Division of Fish Genetics and Biotechnology, Central Institute of Fisheries Education,
    Versova, Mumbai 400 061
  • R R Bhonde, National Center for Cell Science, Ganeshkhind, Pune 411 007.
    National Center for Cell Science, Ganeshkhind, Pune 411 007.

Downloads

Issue

Vol. 52 No. 3 (2005)

Section

Note

License

The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.

How to Cite

Lakra, W. S., Behera, M. R., Sivakumar, N., Goswami, M., & Bhonde, R. R. (2011). Development of cell culture from liver and kidney of Indian major carp, Labeo rohita (Hamilton). Indian Journal of Fisheries, 52(3), 373-376. https://epubs.icar.org.in/index.php/IJF/article/view/7058