In vitro effects of Actinomycin D and Cycloheximide on gonadotropin or 17α-20β-dihydroxy-4- pregnen-3-one-induced maturation of Catla catla


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Authors

  • P K Nayak Central Institute of Freshwater Aquaculture Kausalyaganga, Bhubaneswar - 751002, Orissa, India
  • J Mishra Central Institute of Freshwater Aquaculture Kausalyaganga, Bhubaneswar - 751002, Orissa, India
  • S Ayyappa Central Institute of Freshwater Aquaculture Kausalyaganga, Bhubaneswar - 751002, Orissa, India

Abstract

Actinomycin D and cycloheximide were used as inhibitors to investigate their effect in the oocyte maturation of Catla catla. Full grown immature oocytes incubated with salmon gonadotropin (SG-G100) and 17α-20β-dihydroxy-4- pregnen-3-one (17α-20β-DP) at the concentration of 1 μg/ml induced 91.7 + 1.9 and 93.7 + 2.6 of germinal vesicle breakdown (GVBD) respectively. A significant decline in the frequency of GVBD was observed when the oocytes were incubated with SG-G100 (1 μg/ml) alongwith different concentrations of actinomycin D or cycloheximide. This suggests that both the transcriptional as well as translational inhibitors inhibit the gonadotropin-induced oocyte maturation.
A significant inhibition was also observed when the oocytes were incubated in combination with 17α-20β-DP (1 μg/ml) and different concentrations of cycloheximide. The oocyte maturation was not inhibited when the oocytes were
incubated in the presence of 17α-20β- DP (1 μg/ml) and different concentrations of actinomycin D, which suggests that mRNA synthesis is not obligatory for 17α-20β-DP-induced oocyte maturation. It was also found that the inhibition
of oocyte maturation in cycloheximide treated oocytes somehow extends up to 12 h after which the effects gradually declined

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Author Biographies

  • P K Nayak, Central Institute of Freshwater Aquaculture Kausalyaganga, Bhubaneswar - 751002, Orissa, India
    Central Institute of Freshwater Aquaculture
    Kausalyaganga, Bhubaneswar - 751002, Orissa, India
  • J Mishra, Central Institute of Freshwater Aquaculture Kausalyaganga, Bhubaneswar - 751002, Orissa, India
    Central Institute of Freshwater Aquaculture
    Kausalyaganga, Bhubaneswar - 751002, Orissa, India
  • S Ayyappa, Central Institute of Freshwater Aquaculture Kausalyaganga, Bhubaneswar - 751002, Orissa, India
    Central Institute of Freshwater Aquaculture
    Kausalyaganga, Bhubaneswar - 751002, Orissa, India

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Issue

Vol. 51 No. 1 (2004)

Section

Articles

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How to Cite

Nayak, P. K., Mishra, J., & Ayyappa, S. (2011). In vitro effects of Actinomycin D and Cycloheximide on gonadotropin or 17α-20β-dihydroxy-4- pregnen-3-one-induced maturation of Catla catla. Indian Journal of Fisheries, 51(1), 49-54. https://epubs.icar.org.in/index.php/IJF/article/view/7144