Standardization of latex agglutination test for rapid detection of Aeromonas hydrophila infection


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Authors

  • Rajeshwari Shome Animal Science Section, Central Agricultural Research Institute, Port Blair-744 101, Andaman & Nicobar Islands
  • K K Gaur Animal Health Division, ICAR Research Complex for NEH Region, Umroi Road, Umiam-793 103, Meghalaya
  • B R Shome Animal Health Division, ICAR Research Complex for NEH Region, Umroi Road, Umiam-793 103, Meghalaya
  • Ashok Kumar Dept. of Zoology, BRA University, Agra
  • S Senai Animal Science Section, Central Agricultural Research Institute, Port Blair-744 101, Andaman & Nicobar Islands

Abstract

Diagnostic techniques for rapid detection of pathogens in commercial aquaculture are essential to combat diseases. Latex agglutination test is a simple serological test and has been standardized for the detection of Aeromonas
hydrophila infections in freshwater fish. A total of 48 bacterial cultures were isolated and purified from infected Indian major carps showing either ulceration on the body alone or accompanied by dropsy and degeneration of scales. Out of
48 bacterial cultures tested by standardized LAT, agglutination reaction was observed with 27 cultures. All the 27 LAT positive cultures were also simultaneously confirmed as A. hydrophila by biochemical tests. The LAT negative cultures could be identified either as Gram or oxidase negative or Gram-positive cocci/rods. The study revealed a higher sensitivity and specificity
of the test as all the 27 LAT positive cultures were A. hydrophila.

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Author Biographies

  • Rajeshwari Shome, Animal Science Section, Central Agricultural Research Institute, Port Blair-744 101, Andaman & Nicobar Islands
    Animal Science Section, Central Agricultural Research Institute, Port Blair-744 101,
    Andaman & Nicobar Islands
  • K K Gaur, Animal Health Division, ICAR Research Complex for NEH Region, Umroi Road, Umiam-793 103, Meghalaya
    Animal Health Division, ICAR Research Complex for NEH Region, Umroi Road,
    Umiam-793 103, Meghalaya
  • B R Shome, Animal Health Division, ICAR Research Complex for NEH Region, Umroi Road, Umiam-793 103, Meghalaya
    Animal Health Division, ICAR Research Complex for NEH Region, Umroi Road,
    Umiam-793 103, Meghalaya
  • Ashok Kumar, Dept. of Zoology, BRA University, Agra
    Dept. of Zoology, BRA University, Agra
  • S Senai, Animal Science Section, Central Agricultural Research Institute, Port Blair-744 101, Andaman & Nicobar Islands
    Animal Science Section, Central Agricultural Research Institute, Port Blair-744 101,
    Andaman & Nicobar Islands

Issue

Vol. 51 No. 1 (2004)

Section

Note

License

The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.

How to Cite

Shome, R., Gaur, K. K., Shome, B. R., Kumar, A., & Senai, S. (2011). Standardization of latex agglutination test for rapid detection of Aeromonas hydrophila infection. Indian Journal of Fisheries, 51(1), 103-105. https://epubs.icar.org.in/index.php/IJF/article/view/7172