Assessment of vitellogenin stimulating activity in extracts of brain and sub-oesophageal ganglia of tiger shrimp, Penaeus monodon

Abstract

Studies on the effect of brain and sub-oesophageal ganglionic extracts of Penaeus monodon on the hemolymph vitellogenin concentration of Emerita asiatica showed that the total hemolymph protein level increased, by about 58±3%, at the sixth hour after injection with brain extract of P.monodon. The densitometric scanning and volume analysis of total protein and vitellogenin were carried out using a gel documentation system. The vitellogenin [Relative front value(Rf) =0.1411] in the hemolymph was identified with the relative mobility of yolk protein lipovitellin of the ovary [Rf = 0.1413]. Brain extract injected animals showed a pronounced increase in the hemolymph vitellogenin level by about 210.7±4%, at the sixth hour and sub-oesophageal ganglionic extract induces vitellogenin by about 71.4±2%.