Seroreactivity of somatic soluble proteins of Vibrio alginolyticus
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Authors
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P Sardar
Department of Fishery Pathology and Microbiology, Faculty of Fishery Sciences,
West Bengal University of Animal and Fishery Sciences, Mohanpur - 741 252,
Nadia, West Bengal
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S N Joardar
Department of Fishery Pathology and Microbiology, Faculty of Fishery Sciences,
West Bengal University of Animal and Fishery Sciences, Mohanpur - 741 252,
Nadia, West Bengal
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P Ganesan
Department of Fishery Pathology and Microbiology, Faculty of Fishery Sciences,
West Bengal University of Animal and Fishery Sciences, Mohanpur - 741 252,
Nadia, West Bengal
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T Jawahar Abraham
Department of Fishery Pathology and Microbiology, Faculty of Fishery Sciences,
West Bengal University of Animal and Fishery Sciences, Mohanpur - 741 252,
Nadia, West Bengal
Abstract
Serological characterization of V. alginolyticus was performed which might be quite useful in adopting immunodiagnostic approaches to detect vibriosis in shrimp. The sero-reactivity of V. alginolyticus soluble somatic protein antigen was assessed by agar gel precipitation test (AGPT), counter currentimmunoelectrophoresis and enzyme linked immunosorbent assay (ELISA) using hyperimmune serum against V.alginolyticus, raised in rabbit. SDS-PAGE analysis revealed 21 polypeptides in the range of 15 to 103 kDa in V.alginolyticus somatic soluble antigen preperation. No cross-reactivity of
V.alginolyticus soluble protein antigen was noted with related pathogens, viz. V.harveyi, V. fluvialis and Pseudomonas sp. when counter current immunoelectrophoresis (CIE) was performed. From the present study, it was concluded that seroreactivity exists in V.alginolyticus somatic soluble proteins
that might be exploited in diagnosis of vibriosis in shrimp
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The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.How to Cite
Sardar, P., Joardar, S. N., Ganesan, P., & Abraham, T. J. (2011). Seroreactivity of somatic soluble proteins of Vibrio alginolyticus. Indian Journal of Fisheries, 51(2), 239-244. https://epubs.icar.org.in/index.php/IJF/article/view/7195
