Detection of Aeromonas hydrophila by polymerase chain reaction


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Authors

  • T Raja Swaminathan National Bureau of Fish Genetic Resources, Lucknow - 226 002
  • Gaurav Rathore National Bureau of Fish Genetic Resources, Lucknow - 226 002
  • Rehana Abidi National Bureau of Fish Genetic Resources, Lucknow - 226 002
  • D Kapoor National Bureau of Fish Genetic Resources, Lucknow - 226 002

Abstract

Nine isolates of A. hydrophila isolated from fish and water samples were selected for the present study. The detection of A. hydrophila was done by amplification of the lip gene through known primer sequences at a modified annealing temperature of 650C. A product size of 760 bp was found to be specific for the detection of A. hydrophila by PCR. No amplification product of similar size was obtained in Salmonella typhimurium and Edwardseilla tarda. This
method can be used for the rapid and specific identification of A.hydrophila from aquatic environment

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Author Biographies

  • T Raja Swaminathan, National Bureau of Fish Genetic Resources, Lucknow - 226 002
    National Bureau of Fish Genetic Resources, Lucknow - 226 002
  • Gaurav Rathore, National Bureau of Fish Genetic Resources, Lucknow - 226 002
    National Bureau of Fish Genetic Resources, Lucknow - 226 002
  • Rehana Abidi, National Bureau of Fish Genetic Resources, Lucknow - 226 002
    National Bureau of Fish Genetic Resources, Lucknow - 226 002
  • D Kapoor, National Bureau of Fish Genetic Resources, Lucknow - 226 002
    National Bureau of Fish Genetic Resources, Lucknow - 226 002

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Issue

Vol. 51 No. 2 (2004)

Section

Note

License

The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.

How to Cite

Swaminathan, T. R., Rathore, G., Abidi, R., & Kapoor, D. (2011). Detection of Aeromonas hydrophila by polymerase chain reaction. Indian Journal of Fisheries, 51(2), 251-254. https://epubs.icar.org.in/index.php/IJF/article/view/7198