Characterisation of vitellogenin and vitellin of Fenneropenaeus merguiensis (De Man)


135 / 63

Authors

  • Sherly Zacharia Central Marine Fisheries Research Institue, P.B. No. 1603, Ernakulam, Cochin-682018, India
  • V S Kakati Central Marine Fisheries Research Institue, P.B. No. 1603, Ernakulam, Cochin-682018, India

Abstract

Purification and characterisation of vitellogenin and vitellin from haemolymph and mature ovaries respectively of Fenneropenaeus merguiensis were conducted using ammonium sulphate precipitation technique. The vitellogenin level in
the haemolymph was low in the early stages of development, but increased significantly in the late maturing stages (Stage III) and fell abruptly in spent females. Purified vitellogenin had an approximate molecular weight of 350kDa as determined by PAGE and was characterised as lipoglycoprotein with calcium
affinity on the basis of staining data. Two forms of purified vitellin (Vn1 and Vn2) of molecular weights 550 and 300kDa were also isolated. Vn1 was characterised as a lipo-glyco-calcium complex while Vn2 was glycoprotein in nature based on staining data. SDS PAGE analysis revealed 6 subunits in
purified vitellogenin and 5 subunits in vitellin.

Downloads

Download data is not yet available.

Author Biographies

  • Sherly Zacharia, Central Marine Fisheries Research Institue, P.B. No. 1603, Ernakulam, Cochin-682018, India
    Central Marine Fisheries Research Institue,
    P.B. No. 1603, Ernakulam, Cochin-682018, India
  • V S Kakati, Central Marine Fisheries Research Institue, P.B. No. 1603, Ernakulam, Cochin-682018, India
    Central Marine Fisheries Research Institue,
    P.B. No. 1603, Ernakulam, Cochin-682018, India

Downloads

Issue

Vol. 51 No. 3 (2004)

Section

Articles

License

The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.

How to Cite

Zacharia, S., & Kakati, V. S. (2011). Characterisation of vitellogenin and vitellin of Fenneropenaeus merguiensis (De Man). Indian Journal of Fisheries, 51(3), 255-263. https://epubs.icar.org.in/index.php/IJF/article/view/7199