Induction of triploidy in mrigal, Cirrhinus mrigala by thermal shock treatment of fertilized eggs


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Authors

  • K KarlMarx Fisheries College and Research Institute, Thoothukkudi – 628 008, Tamil Nadu, India.
  • N Sukumaran Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi, Tirunelveli (D.T.), Tamil Nadu
  • Md. Kaleemur Rahman Fisheries College and Research Institute, Thoothukkudi – 628 008, Tamil Nadu, India

Abstract

Triploid mrigal, Cirrhinus mrigala was produced by thermal shock treatment
of eggs five minutes after in vitro fertilization. Cold shock at 12°C, 15°C, and
18°C and heat shock at 40°C were used for the restoration of second polar body in the fertilized eggs. Heat shock for 1 minute duration appeared more effective. Induction of triploidy was confirmed by counting the chromosome number.

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Author Biographies

  • K KarlMarx, Fisheries College and Research Institute, Thoothukkudi – 628 008, Tamil Nadu, India.
    Fisheries College and Research Institute, Thoothukkudi – 628 008, Tamil Nadu,
    India.
  • N Sukumaran, Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi, Tirunelveli (D.T.), Tamil Nadu
    Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar
    University, Alwarkurichi, Tirunelveli (D.T.), Tamil Nadu
  • Md. Kaleemur Rahman, Fisheries College and Research Institute, Thoothukkudi – 628 008, Tamil Nadu, India
    Fisheries College and Research Institute, Thoothukkudi – 628 008, Tamil Nadu,
    India

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How to Cite

KarlMarx, K., Sukumaran, N., & Rahman, M. K. (2011). Induction of triploidy in mrigal, Cirrhinus mrigala by thermal shock treatment of fertilized eggs. Indian Journal of Fisheries, 50(3), 313-317. https://epubs.icar.org.in/index.php/IJF/article/view/7640