Cryopreservation of Penaeus monodon (Fabricius, 1798) spermatozoa.
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Authors
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B Leena Grace
Department of Aquatic Biology and Fisheries, University of Kerala, Kariavattom
Campus, Trivandrum - 695 581
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P Natarajan
Department of Aquatic Biology and Fisheries, University of Kerala, Kariavattom
Campus, Trivandrum - 695 581
Abstract
Mature males of Penaeus monodon were used for the collection of spermatozoa. The cryopreservation technique was carried out in different combinaitons of cryoprotectants using glucose, glycerol, DMSO and methanol. The spermatozoa was preserved at - 196°C and the survival rate was assessed once in five days for a month. The combination of glucose + methanol gave the best survival rate. DMSO was proved to be a moderate cryoprotectant. Combinations of glucose or glycerol gave greater percentage viability. Glycerol alone was also proved to be a good cryoprotectant. Methanol gave least protection to the spermsDownloads
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The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.How to Cite
Grace, B. L., & Natarajan, P. (2011). Cryopreservation of Penaeus monodon (Fabricius, 1798) spermatozoa. Indian Journal of Fisheries, 50(3), 415-419. https://epubs.icar.org.in/index.php/IJF/article/view/7655
