A Simplified procedure for total DNA isolation from coldwater fish, Schizothorax richardsonii (Gray)


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Authors

  • Rajeev Kapila National Research Centre on Coldwater Fisheries, Bhimtal-263 136 (Nainital)
  • D P Mishra Department of Biochemistry, G.B.Pant University of Agriculture and Technology, Pantnagar

Abstract

Isolation of good quality genomic DNA with desired yield is mandatory for conducting
research in molecular biology. Various protocols for isolation of total
DNA from fish tissues have been applied using different buffers, detergents
and enzymes. Under the present investigation a simplified method for total
DNA isolation from muscle tissues of coldwater fish, Schizothorax richardsonii
has been developed using N-Lauroyl sarcosine (sarcosyl) as a detergent. DNA
yield (45μg /100 mg of fresh muscles) obtained by this method was significantly
higher (P<0.05) than the methods using SDS and CTAB as detergents. Furthermore,
homogenization of tissue samples was not needed which further reduced
the chances of mechanical shearing of DNA.

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Author Biographies

  • Rajeev Kapila, National Research Centre on Coldwater Fisheries, Bhimtal-263 136 (Nainital)
    National Research Centre on Coldwater Fisheries, Bhimtal-263 136 (Nainital)
  • D P Mishra, Department of Biochemistry, G.B.Pant University of Agriculture and Technology, Pantnagar
    Department of Biochemistry, G.B.Pant University of Agriculture and Technology,
    Pantnagar

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Issue

Vol. 50 No. 4 (2003)

Section

Note

License

The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.

How to Cite

Kapila, R., & Mishra, D. P. (2011). A Simplified procedure for total DNA isolation from coldwater fish, Schizothorax richardsonii (Gray). Indian Journal of Fisheries, 50(4), 547-551. https://epubs.icar.org.in/index.php/IJF/article/view/7674