Maintenance of haemocytes of edible oyster, Crassostrea madrasensis (Preston) in artificial media


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Authors

  • Gijo Ittoop Central Marine Fisheries Research Institute, Cochin - 682 014, India
  • K C George Central Marine Fisheries Research Institute, Cochin - 682 014, India
  • N K Sanil Central Marine Fisheries Research Institute, Cochin - 682 014, India
  • K S Shobhana Central Marine Fisheries Research Institute, Cochin - 682 014, India
  • P C Nisha Central Marine Fisheries Research Institute, Cochin - 682 014, India

Abstract

The haemocytes of Crassostrea madrasensis were maintained in Hank's balanced
salt solution (HBSS), HBSS with 10% foetal calf serum (FCS), tissue culture
media M 199, and M 199 with 10% FCS. Total counts of viable haemocytes were
taken at 0 hr, 24 hrs, 48 hrs, and 72 hrs. HBSS and HBSS with 10% FCS were
found to maintain cell viability upto 72 hrs without much reduction in the count
of viable cells. In M199 and M199 with 10% FCS, it was found that a substantial
increase of small hyalinocytes occurred at 24 hrs and 48 hrs. This was followed by
a steep fall in the number of viable cells at 72 hrs in both the media. The HBSS,
HBSS with 10% FCS, M199 and M199 with 10% FCS were found to be good for
maintaining the cells in viable state. However, only M 199 and M 199 with 10%
FCS indicated multiplication of haemocytes.

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Author Biographies

  • Gijo Ittoop, Central Marine Fisheries Research Institute, Cochin - 682 014, India
    Central Marine Fisheries Research Institute, Cochin - 682 014, India
  • K C George, Central Marine Fisheries Research Institute, Cochin - 682 014, India
    Central Marine Fisheries Research Institute, Cochin - 682 014, India
  • N K Sanil, Central Marine Fisheries Research Institute, Cochin - 682 014, India
    Central Marine Fisheries Research Institute, Cochin - 682 014, India
  • K S Shobhana, Central Marine Fisheries Research Institute, Cochin - 682 014, India
    Central Marine Fisheries Research Institute, Cochin - 682 014, India
  • P C Nisha, Central Marine Fisheries Research Institute, Cochin - 682 014, India
    Central Marine Fisheries Research Institute, Cochin - 682 014, India

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Issue

Vol. 48 No. 2 (2001)

Section

Articles

License

The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.

How to Cite

Ittoop, G., George, K. C., Sanil, N. K., Shobhana, K. S., & Nisha, P. C. (2011). Maintenance of haemocytes of edible oyster, Crassostrea madrasensis (Preston) in artificial media. Indian Journal of Fisheries, 48(2), 177-182. https://epubs.icar.org.in/index.php/IJF/article/view/8441