Fine structural changes of Rohu {Labeo rohita) sperm after dilution with cryoprotectants


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Authors

  • A Gopalakrishnan National Bureau of Fish Genetic Resources, Canal Ring Road, Telibagh, Lucknow-226 002, UP., India
  • A G Ponniah National Bureau of Fish Genetic Resources, Canal Ring Road, Telibagh, Lucknow-226 002, UP., India
  • Kuldeep K Lal National Bureau of Fish Genetic Resources, Canal Ring Road, Telibagh, Lucknow-226 002, UP., India

Abstract

Spermatozoa of rohu (Laheo rohita) were diluted in two cryodiluents and fine
structural changes were investigated. Damages were observed in head, mid-piece
and tail of about 51% spermatozoa immediately after dilution in one of the
cryodiluents. After 10 minutes of equilibration period, the frequency of damaged
sperms increased to 85%. The other diluent did not exhibit significant structural
changes unlike the former one and nearly 60% sperms remained visibly intact even
after 10 minutes of equilibration time. The present investigation points out (1)
diluents inhibiting sperm motility alone need not always be ideal for a species and
(2) usefulness of ultrastructural images offish sperms in determining the fertilizing
ability of milt and in preliminary screening and selection of cryodiluents for deepfreezing
programmes.

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Author Biographies

  • A Gopalakrishnan, National Bureau of Fish Genetic Resources, Canal Ring Road, Telibagh, Lucknow-226 002, UP., India
    National Bureau of Fish Genetic Resources, Canal Ring Road,
    Telibagh, Lucknow-226 002, UP., India
  • A G Ponniah, National Bureau of Fish Genetic Resources, Canal Ring Road, Telibagh, Lucknow-226 002, UP., India
    National Bureau of Fish Genetic Resources, Canal Ring Road,
    Telibagh, Lucknow-226 002, UP., India
  • Kuldeep K Lal, National Bureau of Fish Genetic Resources, Canal Ring Road, Telibagh, Lucknow-226 002, UP., India
    National Bureau of Fish Genetic Resources, Canal Ring Road,
    Telibagh, Lucknow-226 002, UP., India

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Issue

Vol. 47 No. 1 (2000)

Section

Articles

License

The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.

How to Cite

Gopalakrishnan, A., Ponniah, A. G., & Lal, K. K. (2011). Fine structural changes of Rohu {Labeo rohita) sperm after dilution with cryoprotectants. Indian Journal of Fisheries, 47(1), 21-27. https://epubs.icar.org.in/index.php/IJF/article/view/8562