Extender composition for cryopreservation of Tenualosa ilisha spermatozoa with specific reference to inhibition of motility
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Authors
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Kuldeep K Lal
National Bureau of Fish Genetic Resources, 351128, Dariyapur,
Talkatora Road, Lucknow-226 004, India
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Peyush Punia
National Bureau of Fish Genetic Resources, 351128, Dariyapur,
Talkatora Road, Lucknow-226 004, India
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A G Ponniah
National Bureau of Fish Genetic Resources, 351128, Dariyapur,
Talkatora Road, Lucknow-226 004, India
Abstract
The behaviour of spermatozoa of Tenualosa ilisha in response to commonly usedextenders proved to be different from other fishes studied. The extenders used
for cryopreservation of spermatozoa of various cyprinid and salmonid fishes were
found to be unsuitable for T. ilisha as they activate the sperm on dilution. The
inhibtion point of hilsa sperm motility was assessed by exposing the milt samples
to different concenterations of potassium chloride, ranging from 500 mg to 2500
mg in an extender containing NaCl 5500mg, NaHCOj 20mg, CaClj 20mg, MgSO^
10 mg per 100ml with DMSO @ 10%. At 500 mg/100 ml concentration, spermatozoa
were found inactive in the extended milt and exhibited good motility upon
activation with filtered river water. The milt samples were frozen with seven
extenders composed of base extender containing KCl 1500mg/100ml with additives,
mannitol, BSA, 2% yolk and glucose with 10% DMSO as cryoprotectant. Post thaw
motility after 212 days of cryofreezing indicated that the extender with 2% egg
yolk was superior to others.
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The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.How to Cite
Lal, K. K., Punia, P., & Ponniah, A. G. (2011). Extender composition for cryopreservation of Tenualosa ilisha spermatozoa with specific reference to inhibition of motility. Indian Journal of Fisheries, 46(4), 337-343. https://epubs.icar.org.in/index.php/IJF/article/view/8579
