Application of RAPD in the study of genetic variations between African and American Cichlidae


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Authors

  • D Goldberg MIGAL - Galilee Technological Centre, Kiryat Shmona 10200, Israe
  • K Jackson MIGAL - Galilee Technological Centre, Kiryat Shmona 10200, Israe
  • Y Yehuda MIGAL - Galilee Technological Centre, Kiryat Shmona 10200, Israe
  • Y Plotzky MIGAL - Galilee Technological Centre, Kiryat Shmona 10200, Israe
  • G Degani MIGAL - Galilee Technological Centre, Kiryat Shmona 10200, Israe

Abstract

The DNA of a number of species of the family Cichlidae, found in Africa (Malawi
Lake) and in North America (Mexico), was compared by random amplified
polymorphic DNA (RAPD). The species examined in this study were : Hemichromis
bimaculatus (American cichlid), Pseudotropheus zebra (red dorsal), Cyanotilapia
afra mbamba (red top), Labidochromis flavigulus and Pseudotropheus tropheus
(red cheek). Nine primers were examined in PCR reaction, of which three were
found suitable for use in this technique (ZG4, ZG8 and 118). Band sharing (BS)
within each species with these primers was : 0.00-0.29 in H. bimaculatus, 0.40-
1.00 in L. flavigulus, 0.00-0.25 in P. tropheus, 0.00-1.00 in C. afra and 0.00-0.25
in P. zebra. With all the primers the lowest genetic variation (common bands :
4-8; BS : 0.85-1.00) was found between P. zebra and P. tropheus. A high genetic
variation (common bands : 0-4; BS : 0-0.06) was found between American Cichlidae
(H. bimaculatus) and all the other species (African Cichlidae). The advantage of
this method for measuring variability between species (including hybrids) of the
Cichlidae is discussed.

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Author Biographies

  • D Goldberg, MIGAL - Galilee Technological Centre, Kiryat Shmona 10200, Israe
    MIGAL - Galilee Technological Centre, Kiryat Shmona 10200, Israe
  • K Jackson, MIGAL - Galilee Technological Centre, Kiryat Shmona 10200, Israe
    MIGAL - Galilee Technological Centre, Kiryat Shmona 10200, Israe
  • Y Yehuda, MIGAL - Galilee Technological Centre, Kiryat Shmona 10200, Israe
    MIGAL - Galilee Technological Centre, Kiryat Shmona 10200, Israe
  • Y Plotzky, MIGAL - Galilee Technological Centre, Kiryat Shmona 10200, Israe
    MIGAL - Galilee Technological Centre, Kiryat Shmona 10200, Israe
  • G Degani, MIGAL - Galilee Technological Centre, Kiryat Shmona 10200, Israe
    MIGAL - Galilee Technological Centre, Kiryat Shmona 10200, Israe

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Issue

Vol. 46 No. 3 (1999)

Section

Note

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How to Cite

Goldberg, D., Jackson, K., Yehuda, Y., Plotzky, Y., & Degani, G. (2011). Application of RAPD in the study of genetic variations between African and American Cichlidae. Indian Journal of Fisheries, 46(3), 307-312. https://epubs.icar.org.in/index.php/IJF/article/view/8612