Study of virulence factors of Aeromonas hydrophila isolates causing acute abdominal dropsy and ulcerative diseases in Indian major carps


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Authors

  • Rajeshwari Shome Central Agricultural Research Institute, Port Blair - 744 101 Andaman & Nicobar Islands, India
  • B R Shome Central Agricultural Research Institute, Port Blair - 744 101 Andaman & Nicobar Islands, India
  • Nagesh Ram Central Agricultural Research Institute, Port Blair - 744 101 Andaman & Nicobar Islands, India

Abstract

Six Aeromonas hydrophila isolates from acute abdominal dropsy and
ulcerative disease cases were evaluated for their virulence determinants.
Morphological and biochemical characters' similarity index, ranged from 0.866-
0.978 in relation to the first isolated A. hydrophila (3/95) when 46 parameters
were considered. Similarity index for virulence determinants varied from
0.576-0.846 in similar comparisons taking proteolytic enzymes, amylase,
cytotoxicity, dermonecrotic factor, haemagglutination and hemolysis into
consideration. Generally, the haemagglutination of fish and chicken RBC
appeared closely related to virulence in our isolates. Protease activity and their
thermostability in extracellular preparation in highly virulent and weakly
virulent strains were not significantly different. However, presence of either
thermostable or thermolabile or both types of metalloprotease and serineprotease
seem to be related to virulent strains, but none reflect the actual virulence.
Mannose sensitive and resistant haemagglutination pattern indicates that a
common mechanism does not exist for agglutination of different species and
highly virulent isolates appear to possess different strategies for adherence
and colonization. Western blot analysis showed that all the isolates shared
atleast one common immunogen of 52 KD. However, its significance in relation
to virulence has not been fully established.

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Author Biographies

  • Rajeshwari Shome, Central Agricultural Research Institute, Port Blair - 744 101 Andaman & Nicobar Islands, India
    Central Agricultural Research Institute,
    Port Blair - 744 101 Andaman & Nicobar Islands, India
  • B R Shome, Central Agricultural Research Institute, Port Blair - 744 101 Andaman & Nicobar Islands, India
    Central Agricultural Research Institute,
    Port Blair - 744 101 Andaman & Nicobar Islands, India
  • Nagesh Ram, Central Agricultural Research Institute, Port Blair - 744 101 Andaman & Nicobar Islands, India
    Central Agricultural Research Institute,
    Port Blair - 744 101 Andaman & Nicobar Islands, India

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Issue

Vol. 46 No. 2 (1999)

Section

Articles

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The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.

How to Cite

Shome, R., Shome, B. R., & Ram, N. (2011). Study of virulence factors of Aeromonas hydrophila isolates causing acute abdominal dropsy and ulcerative diseases in Indian major carps. Indian Journal of Fisheries, 46(2), 133-140. https://epubs.icar.org.in/index.php/IJF/article/view/8618