Cryopreservation of spermatophores of the marine shrimp, Penaeus indicus H. Milne Edwards


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Authors

  • A D Diwan Central Marine Fisheries Research Institute, Cochin - 682 014, India
  • Shoji Joseph Central Marine Fisheries Research Institute, Cochin - 682 014, India

Abstract

Attempts on cryopreservation of spermatozoa have been made in penaeidshrimp, Penaeus indicus. Viable spermatozoa could be preserved successfullyfor a period of 60 days at -35°C and -196°C. Among several cryoprotectantsused, a combination of DMSO (5 %) and glycerol (5 %), DMSO (5 %) andtrehalose (0.25M) showed best viability rate (75 to 80 %) in freeze, thawedspermatozoa. Activational changes in spermatozoa during acrosome reactionafter induction with egg water have also been recorded. The importance ofcryogenic storage of spermatozoa is discussed

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Author Biographies

  • A D Diwan, Central Marine Fisheries Research Institute, Cochin - 682 014, India
    Central Marine Fisheries Research Institute, Cochin - 682 014, India
  • Shoji Joseph, Central Marine Fisheries Research Institute, Cochin - 682 014, India
    Central Marine Fisheries Research Institute, Cochin - 682 014, India

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Issue

Vol. 46 No. 2 (1999)

Section

Articles

License

The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.

How to Cite

Diwan, A. D., & Joseph, S. (2011). Cryopreservation of spermatophores of the marine shrimp, Penaeus indicus H. Milne Edwards. Indian Journal of Fisheries, 46(2), 159-166. https://epubs.icar.org.in/index.php/IJF/article/view/8645