Studies on cryogenic preservation of sperm of certain cultivable marine fishes
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Abstract
Significant success has been achieved in long-term cryopreservation of spermat-196°C motility of certain marine fishes like Liza parsia, Sillago sihama,
Mugil cephalus and Gerres oyena. In case of L. parsia sperm motility could
be preserved successfully for more than 240 days using suitable cryoprotectant.
In case of S. sihama and M. cephalus viable sperm could be cryopreserved
for 1 month only, whereas in the case of G. oyena viable sperm could be
preserved for a period of 3 months. While attempting on short-term
preservation of sperm in L. parsia at -10°C, viability could be maintained
for 24 hrs and beyond this at 48 hrs there was total loss of motility. Analysis
of biochemical constituents of seminal plasma in case of L. parsia was carried
out to know the possible causative factors for the loss of motility of sperm and
it was found that the glucose and protein levels decreased significantly in the
seminal fluid cryopreserved for 48 hrs whereas lipid content increased.
Similarly there was drastic reduction in the levels of Na+ and K+ ions in the
seminal fluid cryopreserved for 24 hrs
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The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.How to Cite
Diwan, A. D., & Nandakumar, A. (2011). Studies on cryogenic preservation of sperm of certain cultivable marine fishes. Indian Journal of Fisheries, 45(4), 387-397. https://epubs.icar.org.in/index.php/IJF/article/view/8683