Amplification of shrimp mitochondrial ribosomal genes by polymerase chain reaction


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Authors

  • M S Shekhar Central Institute of Brackishwater Aquaculture, Chennai — 600 008, India
  • P Kishore Chandra Central Institute of Brackishwater Aquaculture, Chennai — 600 008, India

Abstract

The use of genetic markers for population studies requires development of
simple, rapid and reliable molecular genetic techniques. In the present study,
the polymerase chain reaction (PCR) was used in amplifying the mitochondrial
ribosomal DNA (rDNA) genes of 12s and 16s from shrimp muscle tissue. The
size of the amplified portions of rDNA genes was approximately. 415 base pair
(bp) and 520 bp for 12s and 16s respectively. The amplified mitochondrial DNA
by PCR can be used for restriction fragment length polymorphism (RELP)
analysis applicable in studies related to population genetics.

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Author Biographies

  • M S Shekhar, Central Institute of Brackishwater Aquaculture, Chennai — 600 008, India
    Central Institute of Brackishwater Aquaculture, Chennai — 600 008, India
  • P Kishore Chandra, Central Institute of Brackishwater Aquaculture, Chennai — 600 008, India
    Central Institute of Brackishwater Aquaculture, Chennai — 600 008, India

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Issue

Vol. 45 No. 4 (1998)

Section

Note

License

The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.

How to Cite

Shekhar, M. S., & Chandra, P. K. (2011). Amplification of shrimp mitochondrial ribosomal genes by polymerase chain reaction. Indian Journal of Fisheries, 45(4), 433-435. https://epubs.icar.org.in/index.php/IJF/article/view/8693