Enzyme-linked immunosorbent assay and dot immunoassay for detection of Vibrio spp. in tiger shrimp, Peneaus monodon


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Authors

  • S S Mishra Central Institute of Brackishwater Aquaculture, Chennai - 600 008, India
  • M S Shekhar Central Institute of Brackishwater Aquaculture, Chennai - 600 008, India

Abstract

Plate agglutination test, enzyme-linked immunosorbent assay (ELISA) and dot immunoassay (DIA) were standardised and used for detection of Vibrio spp., the causative agents of vibriosis in shrimps. Different bacterial isolates collected from infected shrimps (Penaeus monodon) were tested. Agglutination reaction was noted upto 1:40 dilution of V. parahaemolyticus antiserum. No agglutination was observed with Aeromonas sp. and Pseudomonas sp. Live bacteria followed by formalin killed bacteria, heat-killed bacteria and soluble antigen were found suitable for use as coating antigen in ELISA and DIA. Cross reaction of V. parahaemolyticus antiserum with V. alginolyticus strains and weak reaction with V. anguillarum and V. harveyi were observed with antiserum dilution of 1:1000. However, no cross reaction with Aeromonas sp. and Pseudomonas sp. was noted indicating the specificity of antiserum for detection of Vibrio spp. DIA being more sensitive, quick and economic, has the scope of being used as rapid diagnostic test for detection of vibriosis in shrimps.

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Author Biographies

  • S S Mishra, Central Institute of Brackishwater Aquaculture, Chennai - 600 008, India
    Central Institute of Brackishwater Aquaculture,
    Chennai - 600 008, India
  • M S Shekhar, Central Institute of Brackishwater Aquaculture, Chennai - 600 008, India
    Central Institute of Brackishwater Aquaculture,
    Chennai - 600 008, India

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Issue

Vol. 44 No. 4 (1997)

Section

Articles

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The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.

How to Cite

Mishra, S. S., & Shekhar, M. S. (2011). Enzyme-linked immunosorbent assay and dot immunoassay for detection of Vibrio spp. in tiger shrimp, Peneaus monodon. Indian Journal of Fisheries, 44(4), 369-376. https://epubs.icar.org.in/index.php/IJF/article/view/8824