Effect of zinc mono glycinate and zinc proteinate on production performance, antioxidant profile, metallothionein gene expression and tibia bone parameters in commercial broiler chicken
40 / 31
Authors
-
V.D. LONKAR
KNP College of Veterinary Science Shirwal, Satara -412 801, India.
Author
-
A.S. KADAM
KNP College of Veterinary Science Shirwal, Satara -412 801, India.
Author
-
S.N. JADHAV
KNP College of Veterinary Science Shirwal, Satara -412 801, India.
Author
-
A.A. SRIVASTAVA
Avitech Nutrition Private Limited, Gurugram, Haryana, India.
Author
-
F.N. CHOUDHARI
Avitech Nutrition Private Limited, Gurugram, Haryana, India.
Author
-
N.C. DHORE
KNP College of Veterinary Science Shirwal, Satara -412 801, India.
Author
-
M.R. SHISODIYA
KNP College of Veterinary Science Shirwal, Satara -412 801, India.
Author
Keywords:
Broiler Chicken, Zinc Glycinate, Metallothionein gene, Tibia, AntioxidantAbstract
An experiment was conducted for 35 days on Ven Cobb 430Y strain of broilers (n=180, 3 replicates/group, each comprising 20 birds) to evaluate the effect of inorganic and organic forms of Zinc (Zn). The broilers were randomly divided into three groups viz., T1 (basal diet with inorganic Zn as Zinc Sulfate (ZnS) @ 80g/ton of feed), T2 (basal diet with organic Zn as Zinc Mono Glycinate (ZnGly) @ 27.5g/ton of feed) and T3 (basal diet with organic Zn as Zinc Proteinate (ZnPro) @ 30g/ton of feed). The overall body weight gain (BWG), feed consumption (FC), feed conversion ratio (FCR), and European Production Efficiency Factor (EPEF) were recorded for five weeks. Dressing parameters, antioxidant profile, metallothionein (MT) mRNA gene expression, and tibia parameters were evaluated at the end of the experiment. Supplementation of organic Zn as Zinc Mono Glycinate (ZnGly) @ 27.5g/ton and Zn Proteinates @ 30.0 g/ton of feed was beneficial in improving (p<0.05) FCR without affecting BWG and dressing parameters in broilers. However, EPEF and FC were significantly lower in T3 than T1 and T2. The malondialdehyde (MDA) lipid peroxidation and glutathione peroxidase levels in blood were significantly (p≤0.05) lower and higher, respectively, in T2 than T1, while T2 and T3, as well as T3 and T1, were comparable. Non-significant differences were observed for blood lipid peroxidation/MDA level and plasma total antioxidant capacity between T1, T2, and T3. Percent tibia ash and tibia bone weight were comparable between T1, T2, and T3. However, the tibia Zn content was significantly improved in T2 and T3 than T1. The significantly higher MT mRNA gene expression in liver and duodenal tissue was recorded in T2 and T3 than T1, indicating higher bioavailability of ZnGly and ZnPro than ZnS.
Downloads
References
Ao T., Pierce J.L., Power R., Pescatore A.J., Cantor, A.H., Dawson, K.A. and Ford, M.J. 2009. Effect of different forms of zinc and copper on the performance and tissue mineral content of chicks. Poultry Science, 88: 2171-75.
Ao, T., Pierce, J.L., Pescatore, A.J., Cantor, A.H., Dawson, K.A., Ford, M.J. and Paul, M. 2011. Effects of feeding different concentration and forms of zinc on the performance and tissue mineral status of broiler chicks. British Poultry Science, 52(4): 466-471.
Benzie, I.F. and Strain, J.J. 1999. Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology, 299: 15-27).
Bureau of Indian Standards. 2007. Indian standard poultry feeds-Specification. 5th edn. New Delhi.
Cao, J., Henry, P.R., Davis, S.R., Cousins, R.J., Miles, R.D., Littell, R.C. and Ammerman, C. B. 2002. Relative bioavailability of organic zinc sources based on tissue zinc and metallothionein in chicks fed conventional dietary zinc concentrations. Animal Feed Science and Technology, 101(1-4): 161-170.
De Marco, M., Zoon, M.V., Margetyal, C., Picart, C. and Ionescu, C. 2017. Dietary administration of glycine complexed trace minerals can improve performance and slaughter yield in broilers and reduces mineral excretion. Animal Feed Science and Technology, 232: 182-189.
Drabkin, D.L. and Austin, J.H. (1935). Spectrophotometric studies: II. Preparations from washed blood cells; nitric oxide hemoglobin and sulfhemoglobin. Journal of Biological Chemistry, 112(1): 51-65.
El-Husseiny, O.M., Hashish, S.M., Ali, R.A., Arafa, S.A., Abd El-Samee, L.D. and Olemy, A.A. 2012. Effects of feeding organic zinc, manganese and copper on broiler growth, carcass characteristics, bone quality and mineral content in bone, liver and excreta. International Journal of Poultry Science, 11(6): 368.
Flohe, L. and Gunzier, W.A. 1984. Assay of glutathione peroxidase in Methods in Enzymology (eds. S.P. Colowic and N.O. Kaplan). Academic Press, New York, pp. 114–121
Jahanian, R., Moghaddam, H.N. and Rezaei, A. 2008. Improved broiler chick performance by dietary supplementation of organic zinc sources. Asian-Australasian Journal of Animal Sciences, 21(9): 1348-1354.
Kwiecień, M., Winiarska-Mieczan, A., Milczarek, A., Tomaszewska, E., and Matras, J. 2016. Effects of zinc glycine chelate on growth performance, carcass characteristics, bone quality, and mineral content in bone of broiler chicken. Livestock Science, 191: 43-50.
Liu, Z.H., Lu, L., Li, S.F., Zhang, L.Y., Xy, L., Zhang, K.Y. and Luo, X.G. 2011. Effects of supplemental zinc source and level on growth performance, carcass traits and meat quality of broilers. Poultry Science, 90: 1782-1790.
Mavromati, E., Sena, L., Gjeta, Z. and Mavromati, J. 2018. Assessing the economic efficiency in some broiler farms through the European production efficiency factor (EPEF). European Academic Research, 6(9): 5354-5362.
Placer, Z.A., Cushman, L.L. and Johnson, B.C. 1966. Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Analytical Biochemistry, 16(2): 359-364.
Sahin, K., Smith, M.O., Onderci, M., Sahin, N., Gursu, M.F. and Kucuk, O. 2005. Supplementation of zinc from organic or inorganic source improves performance and antioxidant status of heat-distressed quail. Poultry Science, 84(6): 882-887.
Salim, H.M., Jo, C. and Lee, B.D. 2008. Zinc in broiler feeding and nutrition. Avian Biology Research, 1(1): 5-18.
Snedecor, G.W. and Cochran, W.G. 1994. Statistical Method. 8th edn. The lowa State College Press. Inc. Amer. lowa USA, 950.
Sun, Q., Guo, Y., Ma, S., Yuan, J., An, S. and Li, J. 2012. Dietary mineral sources altered lipid and antioxidant profiles in broiler breeders and post hatch growth of their offsprings. Biological Trace Element Research, 145: 318-324.
Wang, Z.C., Yu, H.M., Xie, J.J., Cui, H., Nie, H., Zhang, T. and Gao, X.H. 2019. Effect of dietary zinc pectin oligosaccharides chelate on growth performance, enzyme activities, Zn accumulation, metallothionein concentration, and gene expression of Zn transporters in broiler chickens. Journal of Animal Science, 97(5): 2114-2124.
Winiarska-Mieczan, A. and Kwiecień, M. 2015. The effects of copper-glycine complexes on chemical composition and sensory attributes of raw, cooked and grilled chicken meat. Journal of Food Science and Technology, 52: 4226-4235.
Zhang, L., Wang, Y.X., Xiao, X., Wang, J.S., Wang, Q., Li, K.X. and Zhan, X.A. 2017. Effects of zinc glycinate on productive and reproductive performance, zinc concentration and antioxidant status in broiler breeders. Biological Trace Element Research, 178: 320-326.
Zhao, J., Shirley, R.B., Vazque-Anon, M., Dibner, J., Richards, J.D., Fishe, P., Hampton, T., Christensen, K.D., Allard, J.P. and Giesen, A.F. 2010. Effects of chelated trace minerals on growth performance, breast meat yield, and footpad health in commercial meat broilers. Journal of Applied Poultry Research, 19: 365-72.
Zhu, X., Shang, X., Lin, G., Li, H., Feng, X., and Zhang, H. 2022. Effects of zinc glycinate on growth performance, serum biochemical indexes, and intestinal morphology of yellow feather broilers. Biological Trace Element Research, 200(9): 4089-4097.
