IN-VITRO ANTIBACTERIAL EFFECT OF COMBINING VITAMIN C WITH ENROFLOXACIN AGAINST QUINOLONE RESISTANT Escherichia coli (EXPEC) ISOLATED FROM BROILER CHICKEN
Abstract views: 50 / PDF downloads: 10
Keywords:
Antimicrobial resistance, Ascorbic acid, Broiler Chickens, Colibacillosis, EnrofloxacinAbstract
Avian pathogenic E. coli (APEC) is the causative agent of colibacillosis in broiler chicken and various antibacterials used to control the infection may lead to the development of multidrug-resistant (MDR) and pan drug-resistant bacteria. Further, antibacterial resistant bacteria also emerge as a result of inappropriate use of antibacterials in chicken, reducing the efficacy of antibacterials used for medical purposes. Keeping this in view, this study was conducted to evaluate the in vitro antibacterial property of vitamin C against resistant bacteria when used along with enrofloxacin. A total of 60 liver swabs collected from the field were used in this study. E. coli were isolated using selective media and were genotypically confirmed by amplification of Adk gene. The resistant genes (qnrA, qnrB and qnrS) were detected through PCR. E. coli isolates were subjected to antimicrobial screening against enrofloxacin and vitamin C (L- ascorbic acid) both by qualitative method and quantitative method. Kirby-Bauer disc diffusion revealed enrofloxacin resistance in 83.33 percent of samples. The MIC of enrofloxacin against resistant isolates was 90.25 μg/ml, but when vitamin C was administered, the MIC was non-significantly lowered to 86.16 μg/ml in the macro broth dilution method. It was also found that vitamin C alone exhibited antibacterial activity at a concentration of ≥ 5 mg/ml. Hence, it is likely that vitamin C may improve antibacterial effects of enrofloxacin in poultry.
Downloads
References
Dho-Moulin, M. and Fairbrother, J.M. (1999). Avian pathogenic Escherichia coli (APEC). Veterinary Research. 30(2- 3):299-316.
Gay, K., Robicsek, A., Strahilevitz, J., Park, C.H., Jacoby, G.A., Barrett, T.J., Medalla, F., Chillera, T.M. and Hooper, D.C. (2006). Plasmid- mediated quinolone resistance in Non-Typhi serotypes of Salmonella enterica. Clinical Infectious Diseases.43 (3):297-304.
Goudarztalejerdi, A., Mohammadzadeh, A., Niazi, K. and Mohammad Mirzaei, M. (2021). High Prevalence of Multidrug Resistance and Biofilm-Formation Ability Among Avian Escherichia coli Isolated from Broilers in Iran. Microbial Drug Resistance. Doi: https://doi.org/10.1089/ mdr.2021.0091.
Grakh, K., D. Mittal, A. Parkash and Y.C. Bangar, 2020. Assessing the potential risk factors associated with avian colibacillosis using a questionnaire survey. Haryana Vet. 59(1): 71-74.
Hooper, D.C. and Jacoby, G.A. (2015). Mechanisms of drug resistance: quinolone resistance. Annals of the New York Academy of Sciences. 1354(1):12.
Kaper, J.B., Nataro, J.P. and Mobley, H.L. (2004). Pathogenic Escherichia coli. Nature Reviews Microbiology. 2(2):123-140.
Kotb, D.N., Mahdy, W.K., Mahmoud, M.S. and Khairy, R.M. (2019). Impact of co-existence of PMQR genes and QRDR mutations on fluoroquinolones resistance in Entero-bacteriaceae strains isolated from community and hospital acquired UTIs. BMC Infectious Diseases. 19(1):1-8.
Rahal, A. and Kumar, A. (2021). Strategies to combat antimicrobial resistance in Indian scenario. The Indian Journal of Animal Sciences. 91(2):79-90.
Ramasamy, T., Keerthana, S., Srinivasan, M.R., Chandrasekar, D., Porteen, K., Borthakur,A., Elamaran,A. and Sriram, P. (2021). Molecular Characterization of Antibiotic Resistance Gene Pattern of Staphylococcus aureus and Escherichia coli in Mastitis Affected Dairy Cows. Indian Journal of Animal Research. 55(4): 463-468.
Seo, K.W. and Lee, Y.J. (2021). Molecular characterization of fluoroquinolone- resistant Escherichia coli from broiler breeder farms. Poultry Science. 101250. doi: https://doi.org/10.1016/j. psj.2021.101250.
Verghese, R.J., Mathew, S.K. and David, A. (2017a). Antimicrobial activity of Vitamin C demonstrated on uropathogenic Escherichia coli and Klebsiella pneumoniae. Journal of Current Research in Scientific Medicine. 3(2):88.
Verghese, R.J., Ramya, S. and Kanungo, R. (2017b). In vitro antibacterial activity of vitamin C and in combination with ciprofloxacin against uropathogenic Escherichia coli. Journal of Clinical & Diagnostic Research. 11:1-5.
Wang, M., Sahm, D.F., Jacoby, G.A. and Hooper, D.C. (2004). Emerging plasmid-mediated quinolone Resistance associated with the qnr gene in Klebsiella pneumoniae clinical isolates in the United States. Antimicrobial Agents Chemotherapy. 48(4): 1295–1299.
Wang, X.M., Liao, X.P., Zhang, W.J., Jiang, H.X., Sun, J., Zhang, M.J., He, X.F., Lao, D.X. and Liu, Y.H. (2010). Prevalence of serogroups, virulence genotypes, antimicrobial resistance, and phylogenetic background of avian pathogenic Escherichia coli in south of China. Foodborne Pathogens and Disease. 7(9): 1099 - 1106.
Wirth, T., Falush, D., Lan, R., Colles, F., Mensa, P., Wieler, L.H., Karch, H., Reeves, P.R., Maiden, M.C., Ochman, H. and Achtman, M. (2006). Sex and virulence in Escherichia coli: an evolutionary perspective. Molecular Microbiology. 60(5): 1136- 51
Zhao, S., Maurer, J.J., Hubert, S., De Villena, J.F., McDermott, P.F., Meng, J., Ayers, S., English, L. and White, D.G. (2005). Antimicrobial susceptibility and molecular characterization of avian pathogenic Escherichia coli isolates. Veterinary Microbiology. 107(3-4):215-224.
Downloads
Submitted
Published
Issue
Section
License
All the copy right belongs to the sponsoring Organization, Tamil Nadu Veterinary and Animal Sciences University, Chennai - 51.