Analysis of Single-Stranded Conformational Polymorphism at 3’UTR of SLC11A1Gene in Jersey Crossbred Cattle
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Authors
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M.Malarmathi
Assistant Professor, Department of Animal Genetics and Breeding, Veterinary College and Research Institute, TANUVAS, Namakkal
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N.Murali
Professor and Head, Department of Animal Genetics and Breeding, Veterinary College and Research Institute, TANUVAS, Namakkal
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R.Saravanan
Assistant Professor, Department of Animal Genetics and Breeding, Veterinary College and Research Institute, TANUVAS, Namakkal
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P.Gopu
Assistant Professor, Department of Animal Genetics and Breeding, Veterinary College and Research Institute, TANUVAS, Namakka
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M. Jeyakumar
Assistant Professor, Department of Animal Genetics and Breeding, Veterinary College and Research Institute, TANUVAS, Namakkal
Keywords:
Disease resistance, SLC11A1 gene, PCR-SSCP, allele frequencies, Jersey crossbred cattleAbstract
Intracellular bacterial diseases cause significant economic losses in livestock industries. Constraints in eradicating infectious diseases indicate the need for selection and breeding of animals based on natural disease resistance. Solute Carrier Family 11 Member 1(SLC11A1) gene expressed in macrophages has significant involvement in innate immune mechanism. Investigation of polymorphism of 3’untranslated region (3’UTR) of SLC11A1 gene was conducted and the Polymerase Chain Reaction - Single-Stranded Conformational Polymorphic (PCR-SSCP) pattern at 3’untranslated region (3’UTR) of SLC11A1 gene in Jersey crossbred cattle was analyzed. A fragment of 175bp encoding 3’UTR was amplified by PCR and the genotyping was done by using PCR-SSCP. SSCP pattern of 3’UTR revealed the presence of four genotypes, viz. AB, BB, CC and CB with a frequency of 0.29, 0.40, 0.17 and 0.13, respectively with allelic frequencies of A (0.144), B (0.615) and C (0.24). The result showed that the population was not in Hardy-Weinberg equilibrium for SLC11A1 gene. SLC11A1 gene was found to be highly polymorphic in the Jersey crossbred cattle population studied.
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