Methane production potential of feed ingredients estimated by  in vitro gas production test

M.Ramachandran; A. Bharathidhasan; V.Balakrishnan

doi:10.56093/ijvasr.v48i1.128390

Authors

M.Ramachandran Professor and Head, Dept of Animal Nutrition Veterinary College and Research Institute, Orathanadu, Thanjavur, TANUVAS
A. Bharathidhasan Associate Professor, Post Graduate Research Institute in Animal Sciences, TANUVAS, Kattupakkam
V.Balakrishnan Professor (Rted), Department of Animal Nutrition, Madras Veterinary College, TANUVAS, Chennai

https://doi.org/10.56093/ijvasr.v48i1.128390

Keywords:

Methane, database, in vitro true digestibility

Abstract

This study was conducted to investigate methane production potential of feed ingredients to develop a database on methane production. Feed ingredients such as cereal grains, cereal by-products and protein supplements were tested for methane production potential using in vitro gas production technique. In vitro true digestibility (IVTD) of cereal grains ranged from 60.1 to 96.7% and oats grain (76.2%) and distiller’s grain (60.1%) had lower (P<0.05) values than other cereal grains. Among the cereal by-products, wheat bran showed highest (P<0.05) IVTD (74.9%) than rice bran (42.7%). IVTD of cottonseed oil cake, black gram and sunflower oil cake was lower (P<0.05) than other protein supplements. Methane production potential of cereal grains at half life (t1/2) ranged from 0.66 to 2.85 ml/100 mg truly digested substrate and the difference was significant (P<0.05), however, maize grain, sorghum grain, bajra and broken rice did not vary among themselves. Average methane production potential of cereal by-products at half life (t1/2) and 24 hrs was 1.27 and 1.81 ml/100 mg truly digested substrate, respectively. Average methane production potential of protein supplements at half life (t1/2) and 24 hrs was 1.39 and 1.75ml/100 mg of truly digested substrate, respectively and the difference was statistically significant (P<0.05). Maximum (P<0.05) methane production potential at half life (t1/2) was recorded for black gram (4.07 ml/100 mg truly digested substrate). Lowest methane production potential both at half life (t1/2) and 24 hrs were recorded in fish meal and spirulina. It can be concluded that among cereal grains, methane production potential was higher (P<0.05) in oats grain at half life (t1/2) and all the cereal grains had similar methane production potential at 24 hrs. Among cereal by-products, wheat bran had higher (P<0.05) methane production potential both at half life (t1/2) and 24 hrs. Among protein supplements, black gram had significantly (P<0.05) higher methane production potential at half life (t1/2) and horse gram had significantly (P<0.05) higher methane production potential at 24 hrs.

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