LDL SUPPLEMENTATION ON VITAL CHARACTERISTICS OF CRYOPRESERVED CANINE SEMEN
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Authors
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Rajput
Postgraduate student, Department of Veterinary Gynaecology and Obstetrics, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University Chennai, TamilNadu, India
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Arunmozhi
Assistant Professor, Department of Veterinary Gynaecology and Obstetrics, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University Chennai, TamilNadu, India
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Rangasamy s
Assistant Professor, Department of Veterinary Gynaecology and Obstetrics, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University Chennai, TamilNadu, India
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Kannan T.A
Professor and Head, Education Cell, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, TamilNadu, India
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Rajalakshmi
Assistant Professor, Department of Veterinary Microbiology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University Chennai, TamilNadu, India
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Sridevi
Professor and Head (Retd.), Department of Veterinary Gynaecology and Obstetrics, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University Chennai, TamilNadu, India
Keywords:
Canine semen, Cryopreservation, LDL, Vital characteristicsAbstract
The present study was aimed at studying the effect of Low-density lipoprotein (LDL) on vital characteristics of canine spermatozoa when compared to semen extended with egg yolk (EY). Sixteen dogs aged between 2-6 years with body condition score of 4-5 on a scale of 0-9 were selected. Semen was collected by digital manipulation and was subjected to pre freeze evaluation. After pre freeze evaluation semen was diluted and cryopreserved by conventional method. Post-thaw evaluation at 24 h revealed highly significant differences in mean percentages of progressive motility, viability, plasma membrane integrity, acrosomal integrity and mitochondrial membrane potential between the semen extended with 20 per cent EY and 8 per cent LDL. However, no significant difference was observed in the mean percentage of sperms showing DNA integrity between the semen extended with 20 per cent EY and 8 per cent LDL at 24 hours post-thaw. This might be due to the detrimental effects of EY on spermatozoa during the process of cryopreservation. It was concluded that LDL has beneficial effects and minimizes the damages caused to spermatozoa during cryopreservation.
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References
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