ISOLATION, IDENTIFICATION AND MOLECULAR DETECTION OF E. coli FROM AN OUTBREAK OF COLIGRANULOMA IN WHITE LEGHORN LAYER CHICKEN


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Authors

  • Venkatesh Yadav J P.V. Narsimha Rao Telangana Veterinary University Rajendranagar, Hyderabad, Telangana State, India
  • Sairam Sandeep G P.V. Narsimha Rao Telangana Veterinary University Rajendranagar, Hyderabad, Telangana State, India
  • Kannaki T. R. ICAR-Directorate of Poultry Research, Hyderabad, Telangana State, India

Keywords:

Colibacillosis, Coligranuloma, E. coli, Layer chicken, PCR

Abstract

Coligranuloma is an economically important bacterial disease caused by Escherichia coli and is characterized by nodular granulomas in liver, mesentery and walls of intestine. Three adult white leghorn layer birds of 24-week-old were brought for necropsy examination from Jeelakarragudem village of West Godavari district. Upon necropsy, the birds revealed typically hard, yellow, nodular granulomas in the mesentery and on the wall of the intestines with few tiny nodular lesions on the liver. Impression smears of the nodules showed large number of gram-negative rod-shaped organisms. The inoculums from the nodular lesions when streaked on to MacConkey agar and EMB agar resulted in characteristic rose-pink and metallic sheen colonies respectively. PCR targeting the E. coli 16S ribosomal DNA resulted in an amplicon of 585 bp. Based on the necropsy lesions, staining and cultural characteristics and PCR, the disease was confirmed as Coligranuloma.

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Submitted

30-11-2022

Published

01-12-2022

How to Cite

J, V. Y., G, S. S., & T. R., K. (2022). ISOLATION, IDENTIFICATION AND MOLECULAR DETECTION OF E. coli FROM AN OUTBREAK OF COLIGRANULOMA IN WHITE LEGHORN LAYER CHICKEN. Indian Journal of Veterinary and Animal Sciences Research, 50(5), 94-98. https://epubs.icar.org.in/index.php/IJVASR/article/view/130950