ISOLATION, IDENTIFICATION AND MOLECULAR DETECTION OF E. coli FROM AN OUTBREAK OF COLIGRANULOMA IN WHITE LEGHORN LAYER CHICKEN
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Authors
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Venkatesh Yadav J
P.V. Narsimha Rao Telangana Veterinary University Rajendranagar, Hyderabad, Telangana State, India
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Sairam Sandeep G
P.V. Narsimha Rao Telangana Veterinary University Rajendranagar, Hyderabad, Telangana State, India
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Kannaki T. R.
ICAR-Directorate of Poultry Research, Hyderabad, Telangana State, India
Keywords:
Colibacillosis, Coligranuloma, E. coli, Layer chicken, PCRAbstract
Coligranuloma is an economically important bacterial disease caused by Escherichia coli and is characterized by nodular granulomas in liver, mesentery and walls of intestine. Three adult white leghorn layer birds of 24-week-old were brought for necropsy examination from Jeelakarragudem village of West Godavari district. Upon necropsy, the birds revealed typically hard, yellow, nodular granulomas in the mesentery and on the wall of the intestines with few tiny nodular lesions on the liver. Impression smears of the nodules showed large number of gram-negative rod-shaped organisms. The inoculums from the nodular lesions when streaked on to MacConkey agar and EMB agar resulted in characteristic rose-pink and metallic sheen colonies respectively. PCR targeting the E. coli 16S ribosomal DNA resulted in an amplicon of 585 bp. Based on the necropsy lesions, staining and cultural characteristics and PCR, the disease was confirmed as Coligranuloma.
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References
Amit-Romach, E., Sklan, D. and Uni, Z.(2004). Microflora ecology of the chicken intestine using 16S ribosomal DNA primers. Poultry Science, 83:1093-1098.
Anon, (1984). Manual of veterinary investigation laboratory techniques. Vol. 1, 3rd Ed., Ministry of Agriculture, Fisheries and Food, London.
Calnek, B.W., Barnes, H.J., Beard, C.W., McDougald, L.R. and Saif, Y.M. (1997). Diseases of Poultry. 10th Ed., Iowa State University Press, Ames, Iowa, USA.
Carli, K.T., Ulan, C.B., Caner, V. and Eyigor, A. (2001). Detection of salmonellae in chicken faeces combination of tetrathionate broth enrichment, capillary PCR, and capillary gel electrophoresis. Journal of Clinical Microbiology, 39(5): 1871-1876.
Charlton, B.R. (2000). Bacterial diseases. In: Avian Disease Manual. 5th Ed., The American Association of Avian Pathologists, USA.
Chauhan, R.S. (2003). Bacterial Diseases. In: Illustrated Special Veterinary Pathology. International Book Distribution Co., UP, India.
DebRoy, C. and Maddox, C.W. (2001). Identification of virulence attributes of gastrointestinal Escherichia coli isolates of veterinary significance. Animal Health Research Reviews, 2:129–140.
Landman, W.J.M. and VanEck, J.H.H.(2017). Coligranulomatosis (Hjarre’s and Wramby’s disease) reconsidered. Avian Pathology, 46(3): 237-241.
Levinson, W. and Jawetz, E. (2001). Medical Microbiology and Immunology, 6thEd., McGraw-Hill International, Toronto.
McLeod, W.G., Rowland, A.C. and Sewell, M.M.H. (1981). Handbook of Tropical Veterinary Laboratory Diagnosis. Centre for Tropical Veterinary Medicine, University of Edinburgh.
Vegad, J.L. and Katiyar, A.K. (2003). Bacterial Diseases. In: A Textbook of Veterinary Special Pathology (Infectious Diseases of Livestock and Poultry). International Book Distribution Co., UP, India.
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