Comparison of endocervical and endometrial cytology to diagnose sub- clinical endometritis in repeat breeding cows


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Authors

  • A. Ganesan Assistant Professor, Department of Veterinary Gynaecology and Obstetrics Veterinary College and Research Institute Tamil Nadu Veterinary and Animal Sciences University Tirunelveli — 627 358, India
  • S. Satheshkumar Professor and Head, Department of Veterinary Gynaecology and Obstetrics, Veterinary College and Research Institute, Orathanadu, Tamil Nadu Veterinary and Animal Sciences University
  • M. Murugan Assistant Professor, Department of Veterinary Gynaecology and Obstetrics, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Tirunelveli — 627 358, India
  • A. Palanisammi Dean, Veterinary College and research Institute, Tirunelveli, Tamil Nadu Veterinary and Animal Sciences University

Keywords:

endocervical cytology, subclinical endometritis, repeat breeding, cross bred cows

Abstract

Repeat breeding due to sub clinical endometritis (SCE) is often diagnosed by cytological examination and the effect of endocervical inflammation (ECI) on increased hazards ofpregnancy is inadequately investigated. In this study, endocervical cytology was compared with the golden standard test `endometrial cytology' to detect SCE as a cause of repeat breeding in cows. Influx of Neutrophils and endocervical inflammation will reflect the status of endometrium and may indirectly reflects the ongoing sub clinical form of endometrial inflammation. Hence, this study was aimed at fixing threshold for PMN in endocervical cytology and comparing the same with golden standard technique. Repeat breeding cows associated with endocervical inflammation with >8% of neutrophils during standing estrus in endocervical cytology will have increased hazards of pregnancy. Our study suggested that a moderate concordance/association between ECI and endometrial inflammation (EDI) in repeat breeding cows, these findings were based on the endocervical and endometrial cytology. Based on the endocervical cytology examination 85% of repeat breeding cow posses EDI (SCE) with a diagnostic sensitivity of 86% and showing moderate clinical acceptability for application of endocervical cytology as diagnostic aid to detect SCE in repeat breeding cows.

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References

Adnane, M., Rachid, K., Christian, H and England, G. (2017). Risk factors of clinical and subclinical endometritis in cattle: A review. Turkish Journal of Veterinary and Animal sciences, 41: 1-11.

Ahmadi, M., Kadivar, R and Vatankhah, A.M. (2016). Evaluation of polymorphonuclear (PMN) cells in cervical sample as a diagnostic technique for detection of subclinical endometritis in dairy cattle. Asian Pacific Journal of Reproduction,1: 1-5.

Barlund, C.S., Carruthers, T.D., Waldner, C.L and Palmer, C.W. (2008). A comparison of diagnostic techniques for postpartum endometritis in dairy cattle. Theriogenology, 69: 714 —723.

Cheong, S.H., Nydam, D.V., Galvao, K.N., Crosier, B.M., Ricci, A and Caixeta, L.S. (2012) Use of reagent test strips for diagnosis of endometritis in dairy cows. Theriogenology, 77(5): 858-864

deBoer, M., LeBlanc, S., Dubuc, J., Meier, S., Heuwieser, W., Sebastian, A., Gilbert, R and Mcdougall, S. (2014). Invited review: Systematic review of diagnostic tests for reproductive-tract infection and inflammation in dairy cows. Journal of Dairy Science, 97: 3983-3999

El-Amrawi and El-Karim(2019). Effect of subclinical endometritis on pro-inflammatory cytokines, prostaglandin-e2, muc-1 and cortisol levels in uterine lavage of repeat breeder dairy cows. Advances in Animal Veterinary Sciences, 7(s2): 1-5.

Fischer, C., Drillich, M., Odau, S., Heuwieser, W., Einspanier, R and Gabler, C. (2010). Selected pro-inflammatory factor transcripts in bovine endometrial epithelial cells are regulated during the oestrous cycle and elevated in case of sub-clinical or clinical endometritis. Reproduction Fertility and Development, 22: 818-829.

Galvao, K.N., Santos, N.R., Galvao, J.S and Gilbert, R.O. (2011). Association between endometritis and endometrial cytokine expression in postpartum Holstein cows. Theriogenology,76:290-299.

Madoz, L., Giuliodori, M., Migliorisi, L., Jaureguiberry, M and de la Sota, R. (2013). Endometrial cytology, biopsy, and bacteriology for the diagnosis of subclinical endometritis in grazing dairy cows. Journal of dairy science, 97: 2013

Melcher., Yvonne., Prunner., Isabella and Drillich, Marc.(2014). Degree of variation and reproducibility of different methods for the diagnosis of subclinical endometritis. Theriogenology, 82: 1016

Sheldon, I. M., Williams, E. J., Aleisha, N. A., Deborah, M. N. and Herath, S. I. (2008). Uterine disease in cattle after parturition. The Veterinary Journal. 176: 115-121.

Sheldon, I.M., Cronin, J., Goetze, L., Donofrio., G. and Schuberth, H. J. (2009). Defining postpartum uterine disease and the mechanisms of infection and immunity in the female reproductive tract in cattle. Biology of Reproduction, 81: 1025-1032.

Smith, D.R., (2009). Veterinary Clinical Epidemiology: A Problem-Oriented Approach. Second Edition. London CRC-Press Ltd, P-59.

Turner, M., Gareth, H and Sheldon, I.M. (2012). Immunity and Inflammation in the Uterus. Reproduction in Domestic Animals, 47: 402-409.

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Submitted

10-01-2023

Published

10-01-2023

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Full Length Articles

How to Cite

A. Ganesan, S. Satheshkumar, M. Murugan, & A. Palanisammi. (2023). Comparison of endocervical and endometrial cytology to diagnose sub- clinical endometritis in repeat breeding cows. Indian Journal of Veterinary and Animal Sciences Research, 49(1), 14-18. https://epubs.icar.org.in/index.php/IJVASR/article/view/132141