STROMAL FIBROPLASIA AS A PROGNOSTIC INDICATOR IN CANINE MAMMARY TUMOURS

Keerthana Santhosh; S. S. Devi; K. S. Prasanna; B. Dhanush Krishna; Laiju M. Philip

doi:10.56093/ijvasr.v52i1.137570

Authors

Keerthana Santhosh M.V.Sc Student, Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy – 680 651, Kerala
S. S. Devi Assistant Professor, Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy – 680 651, Kerala.
K. S. Prasanna Assistant Professor and Head , Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy – 680 651, Kerala
B. Dhanush Krishna Assistant Professor, Department of Veterinary Pathology, CVAS, Pookode
Laiju M. Philip Assistant Professor, Department of Veterinary Surgery and Radiology,, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy – 680 651, Kerala

https://doi.org/10.56093/ijvasr.v52i1.137570

Keywords:

Canine mammary tumours, collagen, fibroplasia, stroma

Abstract

Mammary tumours are considered as the most important neoplasms in dogs especially in sexually intact female dogs. Prognosis is directly associated with various factors such as tumour size, metastases, involvement of lymph node, tumour type and grade. Recently, tumour microenvironment (TME) has gained huge importance and proved to be a key regulator of breast cancer's biologic behaviour, according to mounting data. The capacity of extracellular matrix components like collagen to alter their intrinsic structure and alignment aids in tumour invasion and metastasis. We present a case study of 25 canine mammary tumours (CMTs), where the stroma associated with the malignancy was specifically considered while histomorphologically analysing the tumours. The majority of the tumours were malignant (96 per cent) and one was identified to be benign. Simple carcinomas made up the majority of tumours, followed by mixed types. Cancer associated stroma was assessed by routine H and E staining and additionally by performing immunohistochemistry technique with anti-cytokeratin antibody to access the relative abundance of neoplastic epithelial cells. Special stains such as Picrosirius red, Masson’s trichrome and Herovici were employed to demonstrate the desmoplastic stromal reaction and varying grades of collagen could be observed in the stroma of different tumours. The extent of fibroplasia was found to be directly proportional to the grade and size of tumour. Thus, results of the present study indicated that the cancer associated stroma significantly in fluence the CMT progression and clinical outcome.

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