AN IN SILICO APPROACH FOR THE EVALUATION OF CELL WALL COMPONENTS OF PROBIOTICS AS BIOLOGICAL ADSORBERS OF  AFLATOXINS

P. Jalantha; Ganne Venkata Sudhakar Rao; N. Pazhanivel; M. Parthiban; P. Veeramani; M.R. Srinivasan; C. Soundararajan

doi:10.56093/ijvasr.v52i2.137814

Authors

P. Jalantha Assistant Professor, Laboratory Animal Medicine Unit Director, Centre for Animal Health Studies, TANUVAS, Madhavaram Milk Colony, Chennai – 600 051
Ganne Venkata Sudhakar Rao Professor and Head, Department of Veterinary Pathology, Madras Veterinary College, Chennai -7
N. Pazhanivel Professor, Department of Veterinary Pathology, Madras Veterinary College, TANUVAS, Chennai -7
M. Parthiban Professor, Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai -7
P. Veeramani Associate Professor, Education Cell, Madras Veterinary College, Chennai -7
M.R. Srinivasan Assistant Professor, Laboratory Animal Medicine Unit Director, Centre for Animal Health Studies, TANUVAS, Madhavaram Milk Colony, Chennai – 600 051
C. Soundararajan Director, Centre for Animal Health Studies, TANUVAS, MMC, Chennai -51

https://doi.org/10.56093/ijvasr.v52i2.137814

Keywords:

Aflatoxicosis, biological binder, in silico, probiotics

Abstract

Aflatoxins are produced in poultry feed by two major fungal species viz., Aspergillus flavus and Aspergillus parasiticus during hot and humid seasons. To detoxify these aflatoxins, various binders are being used in the field which are mostly synthetic compounds with possible other toxicities, the harmless beneficial probiotics are being considered as binders to neutralize the effect of aflatoxins in the feed. Bacterial organisms (probiotics) like Lactobacillus rhamnosus, Lactobacillus casei, Bacillus subtilis, Enterococcus faecium and yeast (Saccharomyces cerevisiae) were selected and their cell wall structures have been retrieved and molecular docking was performed against four types of aflatoxins namely AFB1, AFB2, AFG1 and AFG2. The results revealed that cell wall components of the chosen bacterial organisms have a good binding affinity towards four types of aflatoxins. Based on this in silico results, it is evident that the both the lipoteichoic acid (LTA) and wall teichoic acid (TA) in the cell wall of probiotic organisms is responsible for its binding against aflatoxins.

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