Studies on fresh milt parameters and cryopreservation of spermatozoa of endangered, golden mahseer, tor putitora (hamilton)


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Authors

  • Ravindragouda Patil Fisheries Research and Information Center, KVAFSU, Hessaraghatta, Bengaluru- 560 089, Karnataka State
  • Wazir Singh Lakra Central Institute of Fisheries Education, Versova, Off Yari Road, Seven Bungalows, Andheri (W), Mumbai, Maharastra
  • Shrinivas Jahageerdar Central Institute of Fisheries Education, Versova, Off Yari Road, Seven Bungalows, Andheri (W), Mumbai, Maharastra
  • Gopal Krishna Central Institute of Fisheries Education, Versova, Off Yari Road, Seven Bungalows, Andheri (W), Mumbai, Maharastra
  • Asim Kumar Pal Central Institute of Fisheries Education, Versova, Off Yari Road, Seven Bungalows, Andheri (W), Mumbai, Maharastra

Keywords:

Tor putitora, fresh milt, parameters, NBT, cryopreservation

Abstract

Physico-chemical parameters of fresh milt and mitochondrial activity of fresh spermatozoa of the endangered golden mahseer, Tor putitora (Hamilton) were investigated. Cryopreservation and fertility evaluation of cryopreserved-thawed spermatozoa of T. putitora were carried out. The concentration of spermatozoa and the spermatocrit values of the fresh milt were estimated to be 3.96±0.12 x 107 spermatozoa/ml and 70.00±1.53% respectively. The motility percentage of spermatozoa in the fresh milt was estimated to be 94.84±0.43. The elemental composition analysis revealed that the concentration of K+ was higher i.e., 14.13±0.32 mg/l when compared to that of Na+, Ca+, Mg+ and Zn+. The concentration of total reducing sugars and total proteins were estimated to be 51.79±0.47mg/100 ml and 40.57±0.75 mg/100ml respectively. The absorbance value of the fresh spermatozoa in Sperm Mitochnodrial Activity Index (SMAI) assessment by Nitro Blue Tetrazolium (NBT) assay was estimated to be 0.33±0.012. The optimum sperm: egg ratio was estimated to be 3.96 ±0.12 x 104+ no./egg. Hatching percentage of 45.97±1.72 was recorded for the cryopreserved-thawed  spermatozoa while the fresh milt control recorded a hatching percentage of 73.10±0.82.

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References

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Submitted

20-07-2023

Published

07-11-2023

Issue

Vol. 45 No. 1 (2016): IJVASR Jan - Feb 2016

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Full Length Articles

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All the copy right belongs to the sponsoring Organization, Tamil Nadu Veterinary and Animal Sciences University, Chennai - 51.

How to Cite

Ravindragouda Patil, Wazir Singh Lakra, Shrinivas Jahageerdar, Gopal Krishna, & Asim Kumar Pal. (2023). Studies on fresh milt parameters and cryopreservation of spermatozoa of endangered, golden mahseer, tor putitora (hamilton). Indian Journal of Veterinary and Animal Sciences Research, 45(1), 460-470. https://epubs.icar.org.in/index.php/IJVASR/article/view/139515