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  • B. Behera Ph.D. Scholar, Department of Veterinary Pathology, Madras Veterinary College, TANUVAS, Chennai - 600 007
  • N. Pazhanivel Professor, Department of Veterinary Pathology, Madras Veterinary College, TANUVAS, Chennai- 600 007
  • S. Vairamuthu Professor and Head, Centralised Clinical Laboratory, Madras Veterinary College, Chennai-600 007
  • S. Sureshkannan Professor and Head, Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, TANUVAS, Chennai - 600 007
  • T.M.A. Senthil Kumar Professor and Head, Zoonosis Research Laboratory, TANUVAS, MMC, Chennai – 51
  • P. Jalantha Assistant Professor, Laboratory Animal Medicine Unit, DCAHS, TANUVAS, MMC, Chennai - 51
  • Ganne Venkata Sudhakar Rao Professor and Head, Department of Veterinary Pathology, Madras Veterinary College, TANUVAS, Chennai - 600 007


Custard apple, Haemolytic Assay, In-vitro, Methanolic Extract, Stinkweed


Many plants are being used as food additives and also as traditional medicine because of their preservative and curative effects. Various phytochemicals from medicinal plants like polyphenols, flavonoids, and Vitamin C serve as lead compounds in drug design and discovery. These active compounds are used as an alternative medicine to treat diseases like cancer, cardiovascular, Alzheimer’s, and Parkinson’s disease. Plant extracts can positively affect the red cell membrane and many plants have serious adverse effects, which include induction of haemolytic anaemia. Therefore, many of the commonly used plants need to be evaluated for their potential haemolytic activity and it represents an important starting point in this regard. The haemolytic activity of any compound is the ultimate indicator of general cytotoxicity towards normal healthy cells. Methanolic extract of both Cleome gynandra plant (Stinkweed) and Annona squamosa (Custard apple) seeds were prepared. The haemolytic assay was conducted to check the haemolytic activity of both extracts as well as their combination. The study revealed that Cleome gynandra plant extract had the least haemolytic activity followed by Annona squamosa seed extract and a combination of both extracts. But the haemolytic activity increases when the concentration of all extract increases. 


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IN-VITRO STUDY OF HAEMOLYTIC ASSAY FOR METHANOLIC EXTRACT OF Cleome gynandra PLANT (STINKWEED) AND Annona squamosa (CUSTARD APPLE) SEED . (2023). Indian Journal of Veterinary and Animal Sciences Research, 52(5), 34-41.