HPLC AND SPECTROPHOTOMETRIC ANALYSIS OF TETRACYCLINE RESIDUES IN MARKETED PORK OF ASSAM
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Authors
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D.C. Roy
Department of Pharmacology & Toxicology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-781022.
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R. Gogoi
Department of Pharmacology & Toxicology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-781022.
Keywords:
HPLC, Spectrophotometric, Tetracycline and marketed porkAbstract
The present study was undertaken to detect residues of Tetracycline in marketed pork using a High Performance Liquid Chromatography (HPLC) System and a UV-Vis Spectrophotometer. 300 samples of marketed pork were collected for the study. The samples after collection were preserved at -20°C. Analyses of the samples using High Performance Liquid Chromatography with UV-Vis Detector were done as per the method of Oka et al., 1985 while analyses of the same samples using UV-Vis Spectrophotometer were done as per the method of Yongnianian et al, 2010. Recovery ranged from 85-93% (HPLC) and 68-83% (Spectrophotometer). Out of the tested samples, 4 samples were detected to be positive for trace residues of tetracycline using Spectrophotometer while 6 samples were detected for tetracycline residues using HPLC method which were well below the Maximum Residue Limit (MRL) value. The method of HPLC is more sensitive than the Spectrophotometric one. Thus, the HPLC method is useful for monitoring of tetracycline residues in pork.
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References
Anonymus, Report on Integrated Sample survey for estimation of production of Milk, Egg and Meat, Directorate of Animal Husbandry & Veterinary Department, Govt. of Assam, 2008-09
Biswas, A.K.; Rao, G.S.; Kondaiah, N.; Anjaneyulu, A.S.R.; Mendiratta, S.K.; Prasad, R. and Malik, J.K. (2007). J. Food and Drug Anal. 15(3):278-284.
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Shahid, M.A.; Siddique, M; Abubakar, M; Arshed, M.J.; Asif, M. and Ahmad, A.(2007). Asian Journal Poultry Science ,1(1):8-15.
Yongnian N., Na D. and Serge K. (2010). Anal. Methods. 2 : 1302-1309
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