EFFECT OF OPU SESSION AT INDUCED AND RANDOM STAGES OF THE OVARIAN CYCLE ON OOCYTE YIELD AND IN-VITRO EMBRYO PRODUCTION IN ONGOLE AND GIR HEIFERS

B. Kalyani; N. Raghavendra Srikanth; K. Sunny Praveen; K. Anusha; M. Mutha Rao

doi:10.56093/ijvasr.v54i5.172392

Authors

B. Kalyani MVSC Scholar, Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati - 517 502. Andhra Pradesh
N. Raghavendra Srikanth Scientist, Livestock Research Station, Lam, Guntur - 522 034
K. Sunny Praveen Scientist, Livestock Research Station, Lam, Guntur - 522 034
K. Anusha Associate Professor and Head, Veterinary Clinical Complex, College of Veterinary Science, Proddatur - 516 360
M. Mutha Rao Professor and Officer in Charge, Livestock Research Station, Lam. Guntur - 522 034

https://doi.org/10.56093/ijvasr.v54i5.172392

Keywords:

Bos indicus, OPU, COC recovery, in-vitro embryo production

Abstract

The present study was carried out to evaluate the effect of OPU session i.e OPU performed at known stage of cycle (OPU1) vs OPU performed at unknown stage of cycle (OPU2), on oocyte yield and in-vitro embryo production in two different Bos indicus breeds i.e., Ongole (n=6) and Gir (n=6) heifers, aged 2-4 years. OPU 1 was carried out on day 9 (luteal phase) of induced cycle and after a resting period of 3-4 weeks OPU2 was performed at random stage of cycle. A total of 24 OPU sessions were performed in 12 animals. The mean antral follicle count (AFC) (32.58±2.22 and 30.50±2.16), cumulus oocyte complexes (COC) recovery (24.58±2.50and 22±1.95), viable oocyte yield (21.25±2.20and 19.33±1.72) and embryos produced (5.91±0.9and 6.66±1.13) in OPU 1 and OPU 2 respectively,did not show significant variation (P>0.05). Similarly the breed of the oocyte donor had not shown any effect on OPU-IVEP parameters. The results suggest that the stage of estrous cycle did not impact the efficacy of OPU. Ongole and Gir heifers, both being Bos indicus category, performed uniformly in both OPU sessions.

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References

Adams, G.P., Evans, A.C.O. and Rawlings, N.C. (1994). Follicular waves and circulating gonadotrophins in 8-month-old prepubertal heifers. Reproduction, 100(1): 27-33.

Batista, E.O.S., Macedo, G.G., Sala, R.V., Ortolan, M.D.D.V., Sá Filho, M.F.D., Del Valle, T.A., Jesus, E F., Lopes, R.N.V.R., Renno, F.P. and Baruselli, P.S. (2014). Plasma antimullerian hormone as a predictor of ovarian antral follicular population in Bos indicus (Nelore) and Bos taurus (Holstein) heifers, Reproduction in Domestic Animals, 49(3): 448-452.

Blondin, P., Bousquet, D., Twagiramungu, H., Barnes, F. and Sirard, M.A. (2002). Manipulation of follicular development to produce developmentally competent bovine oocytes. Biology of reproduction, 66(1): 38-43.

Bols, P.E.J. (1997). Transvaginal ovum pick-up in the cow, technical and biological modifications. Doctoral thesis, submitted to University of Ghent, Belgium.

Bousquet, D., Twagiramungu, H., Morin, N., Brisson, C., Carboneau, G., and Durocher, J. (1999). In vitro embryo production in the cow: an effective alternative to the conventional embryo production approach. Theriogenology, 51(1): 59-70.

Burns, D.S., Jimenez-Krassel, F., Ireland, J.L., Knight, P.G. and Ireland, J.J. (2005) Numbers of antral follicles during follicular waves in cattle: evidence for high variation among animals, very high repeatability in individuals, and an inverse association with serum follicle-stimulating hormone concentrations. Biology of Reproduction, 73: 54-62.

Cavalieri, F.L.B., Morotti, F., Seneda, M.M., Colombo, A.H.B., Andreazzi, M.A., Emanuelli, I.P. and Rigolon, L.P. (2018). Improvement of bovine in vitro embryo production by ovarian follicular wave synchronization prior to ovum pick-up. Theriogenology, 117: 57-60.

Chaubal, S.A., Ferre, L.B., Molina, J.A., Faber, D.C., Bols, P.E.J., Rezamand, P., Tian, X. and Yang, X. (2007). Hormonal treatments for increasing the oocyte and embryo production in an OPU-IVP system, Theriogenology, 67: 719-728.

Chaubal, S.A., Molina, J.A., Ohlrichs, C.L., Ferre, L.B., Faber, D.C., Bols, P.E.J., Riesen, J.W., Tian, X. and Yang, X. (2006). Comparison of different transvaginal ovum pick-up protocols to optimize oocyte retrieval and embryo production over a 10-week period in cows, Theriogenology, 65: 1631-1648.

De Lima Moschini, G.A., Gaitkoski, D., de Almeida, A.B.M., Hidalgo, M.M.T., Martins, M.I.M., Blaschi, W. and Barreiros, T.R.R. (2021). Comparison between in-vitro embryo production in Bos indicus and Bos taurus cows. Research, Society and Development, 10(7).

Ferret, S., Grimard, B., Ponter, A.A., Joly, C., Ponsart, C., Humblot, P. (2006) Reduction of body-weight gain enhances in vitro embryo production in overfed superovulated dairy heifers. Reproduction, 131(4):783-794.

Galli, C., Crotti, G., Notari, C., Turini, P., Duchi, R. and Lazzari, G. (2001). Embryo production by ovum pick up from live donors. Theriogenology, 55(6): 1341-1357.

Garcia, J.M., Puelker, R.Z., Avelino, K.B., Vantini, R., Rodrigues, C.F.M. and Esper, C.R. (2000). Factors affecting embryo production-effect of follicular wave synchronization method on an ovum pick-up program. Theriogenology, 53(1): 354-354.

Getz, I. (2004). The efficiency of ovarian stimulation in cows used for transvaginal ultrasound aspiration of oocytes and culture in vitro of bovine embryos (Doctoral dissertation, Dissertation. Faculty of Veterinary Medicine. Zagreb, Croatia).

Ginther, O.J., Baldrighi, J.M., Siddiqui, M.A.R., and Araujo, E.R. (2016). Complexities of follicle deviation during selection of a dominant follicle in Bos taurus heifers. Theriogenology, 86(8): 2012-2019.

Gobikrushanth, M., Purfield, D.C., Colazo, M.G., Butler, S.T., Wang, Z. and Ambrose, D.J. (2018). The relationship between serum anti-müllerian hormone concentrations and fertility, and genome-wide associations for anti-müllerian hormone in Holstein cows. Journal of dairy science, 101(8): 7563-7574.

Hendriksen, P.J.M., Vos, P.L.A.M., Steenweg, W.N.M., Bevers, M.M. and Dieleman, S.J. (2000). Bovine follicular development and its effect on the in vitro competence of oocytes. Theriogenology, 53(1): 11-20.

Karadjole, M., Getz, I., Samardzija, M., Matkovic, M., Makek, Z., Karadjole, T., Bacic, G., Macesi, N. and Dobranic, T. (2007). The effect of origin of the immature oocytes on in- vitro developmental competence of bovine embryos. Proceedings of the 23rd Annual Meeting of European Embryo Transfer Association, 7-8. Alghero, Italy, 180.

Kruip, T.A., Pieterse, M.C., Van Beneden, T.H., Vos, P.L., Wurth, Y.A. and Taverne, M.A. (1991). A new method for bovine embryo production: a potential alternative to superovulation. The Veterinary Record, 128(9): (208- 210).

Looney, C.R., Lindsey, B.R., Gonseth, C.L. and Johnson, D.L. (1994). Commercial aspects of oocyte retrieval and in-vitro fertilization (IVF) for embryo production in problem cows. Theriogenology, 41: 67-72.

Machatkova, M., Krausova, K., Jokesova, E. and Tomanek, M. (2004). Developmental competence of bovine oocytes: effects of follicle size and the phase of follicular wave on in vitro embryo production. Theriogenology, 61(2-3): 329-335.

Majerus, V., De Roover, R., Etienne, D., Kaidi, S., Massip, A., Dessy, F. and Donnay, I. (1999). Embryo production by ovum pick up in unstimulated calves before and after puberty. Theriogenology, 52(7): 1169-1179.

Malhi, P.S., Adams, G.P., Pierson, R.A. and Singh, J. (2006). Bovine model of reproductive aging: response to ovarian synchronization and superstimulation. Theriogenology, 66(5): 1257-1266.

Merton, J.S., Otter, T., Aerts, B. and Mullaart, E. (2008). Effect of oocyte collection method and breed on efficiency of oocyte collection and subsequent in vitro bovine embryo production. Proceedings association, 12-13 September. Pau, France.

Mullaart, E., Verbrugge, A., Aerts, B. and Merton, J.S. (1999). Optimization of OPU procedure. Proceedings of the 15th scientific meeting of European embryo transfer association, 10-11 September. Lyon, France.pp.216.

Petyim, S., Båge, R., Hallap, T., Bergqvist, A.S., Rodrıguez-Martınez, H. and Larsson, B. (2003). Two different schemes of twice-weekly ovum pick- up in dairy heifers: effect on oocyte recovery and ovarian function. Theriogenology, 60(1): 175-188.

Pontes, J.H.F., Melo Sterza, F.A., Basso, A.C., Ferreira, C.R., Sanches, B.V., Rubin, K.C.P. and Seneda, M.M. (2011). Ovum-pick up, in-vitro embryo production, and pregnancy rates from a large scale commercial program using Nelore cattle (Bos indicus) donors. Theriogenology, 75: 1640-1646.

Pontes, J.H.F., Silva, K.C.F., Basso, A.C., Rigo, A.G., Ferreira, C.R., Santos, G.M.G. and Sterza, F.A.M. (2010). Large-scale in-vitro embryo production and pregnancy rates from Bos taurus, Bos indicus, and indicus- taurus dairy cows using sexed sperm. Theriogenology, 74(8): 1349-1355.

Praveen (2024). Antral follicle count and serum anti-mullerian hormone levels vis-à-vis in-vitro embryo production in Ongole (Bos indicus) cows. Thesis submitted to Sri Venkateswara Veterinary University, Tirupati, A.P.

Presicce, G. ., Jiang, S., Simkin, M., Zhang, L., Looney, C.R., Godke, R.A. and Yang, X. (1997). Age and hormonal dependence of acquisition of oocyte competence for embryogenesis in prepubertal calves. Biology of Reproduction, 56: 386-392.

Rajkumar (2023). Comparative efficacy of OPU and in-vitro embryo production between Gir and Ongole (Bos indicus) donors. Thesis submitted to Sri Venkateswara Veterinary University, Tirupati, A.P.

Sales, J.N.D.S., Iguma, L.T., Batista, R.I.T.P., Quintão, C.C.R., Gama, M.A.S., Freitas, C.D. and Baruselli, P.S. (2015). Effects of a high-energy diet on oocyte quality and in vitro embryo production in Bos indicus and Bos taurus cows. Journal of dairy science, 98(5): 3086-3099.

Santos Dos, G.M.G., Silva-Santos, K.C., Barreiros, T.R.R., Morotti, F., Sanches, B.V., de Moraes, F.L.Z., Blaschi, W. and Seneda, M.M. (2016). High numbers of antral follicles are positively associated with in vitro embryo production but not the conception rate for FTAI in Nelore cattle. Animal Reproduction Science, 165: 17-21.

Scott, C.A., Robertson, L., de Moura, R.T., Paterson, C. and Boyd, J.S. (1994). Technical aspects of transvaginal ultrasound-guided follicular aspiration in cows. The Veterinary Record, 134(17): 440-443.

Sreemannarayana, T., Srikanth, N.R. and Kurati, S.P. (2024). Effect of FSH pre-stimulation on oocyte recovery in Ongole (Bos indicus) cows. Indian Journal of Veterinary and Animal Sciences Research, 53(4): 22-31.

Taneja, M., Bols, P.E., Van de Velde, A., Ju, J.C., Schreiber, D., Tripp, M.W., Levine, H., Echelard, Y., Riesen, J. and Yang, X. (2000). Developmental competence of juvenile calf oocytes in-vitro and in-vivo: influence of donor animal variation and repeated gonadotropin stimulation. Biology of Reproduction, 62: 206-13.

Vieira, L.M., Rodrigues, C.A., Netto, C.A., Guerreiro, B.M., Silveira, C.R., Moreira, R.J.C., Sá Filho, M.F., Bó, G.A., Mapletoft, R.J. and Baruselli, P.S. (2014). Superstimulation prior to the ovum pick-up to improve in- vitro embryo production in lactating and non-lactating Holstein cows. Theriogenology, 82:318-2