PHYTOCHEMICAL CHARACTERIZATION AND EVALUATION OF ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF MANGIFERA INDICA LEAF EXTRACT


21 / 13

Authors

  • I. Manikkavasagan Assistant Professor, Department of Food Safety and Quality Assurance, College of Food and Dairy Technology, TANUVAS, Koduvalli, Chennai - 600 052.
  • K. Vijayarani Professor and Head (Retd.), Bioinformatics Centre, MVC, Chennai - 600 007
  • B. Murugan Professor and Head, Department of Food Safety and Quality Assurance, College of Food and Dairy Technology, Koduvalli, Chennai - 52.
  • S. Meignanalakshmi Professor, Department of Animal Biotechnology, Madras Veterinary College, Chennai
  • S. Eswari Professor and Head, Centre for Stem Cell Research and Regenerative Medicine, MVC, Chennai - 600 007
  • V. Ragavendiran PG Scholar, College of Food and Dairy Technology, TANUVAS, Koduvalli, Chennai - 600 052

https://doi.org/10.56093/ijvasr.v54i5.172412

Keywords:

Mangifera indica, GC-MS, antibacterial activity, antioxidant activity

Abstract

Mango (Mangifera indica) is a leading tropical fruit crop that is grown worldwide. Different parts of the mango fruit have traditionally been used for various medicinal  applications.  The  current  study  explored  the  phytochemical  components of mango leaf extract and examined its antibacterial and antioxidant properties. Phytochemical screening revealed the presence of flavonoids, phenols, steroids, terpenoids, coumarins, glycosides, saponins, and tannins in the extracts. Gas chromatography-mass spectrometry (GC-MS) analysis revealed the presence of several bioactive compounds in the extract. The antibacterial activity of the leaf extract showed dose-dependent inhibition  against  Escherichia  coli  (11–8  mm),  Staphylococcus  aureus  (13–8  mm), and Salmonella enterica (11–7 mm). The extract also exhibited dose-dependent DPPH radical scavenging activity, reflecting its strong antioxidant potential. Therefore, the ethanolic leaf extract of Mangifera indica has considerable antioxidant and antimicrobial activities,  implying  its  potential  use  in  the  food  and  pharmaceutical  industries.

Downloads

Download data is not yet available.

References

Akash Dagale, M.H. (2025). Phytochemical and anti-microbial analysis of Mangifera indica leaves. International Journal of Pharmaceutical Sciences. doi:10.5281/ZENODO.15365302.

Basavarajappa, D.S., Kumar, R.S., Almansour, A.I., Chakraborty, B., Bhat, M.P., Nagaraka, S.K., Hiremath, H., Perumal, K. and Nayaka, S. (2022). Biofunctionalized Silver nanoparticles synthesized from Passiflora vitifolia leaf extract and evaluation of its antimicrobial, antioxidant and anticancer activities. Biochemical Engineering Journal, 187:108517. doi:10.1016/j.bej.2022.108517.

Bharath, G., Banat, F., Show, P.L. and Cocoletzi, H.H. (2019). Mango leaf extract incorporated chitosan antioxidant film for active food packaging. International Journal of Biological Macromolecules, 126:1234–1243. doi:10.1016/j. ijbiomac.2018.12.196.

Deependra, Y., Pal, A.K., Singh, S.P. and Sati, K. (2022). Phytochemicals in mango (Mangifera indica) parts and their bioactivities: A Review. Crop Research, 57(1):79–95.

Ediriweera, M.K., Tennekoon, K.H. and Samarakoon, S.R. (2017). A review on ethnopharmacological applications, pharmacological activities, and bioactive compounds of Mangifera indica (Mango). Evidence-Based Complementary and Alternative Medicine: eCAM, p.6949835. doi:10.1155/2017/6949835.

Ghosh, B., Majumder, S., Acharyya, S., Ghosh, A., Saha, S., Sarkar, S., Chakraborty, S. and Bhattacharya, M. (2022). Comparative phytochemical analysis of mature mango leaves from nineteen cultivars of Murshidabad district, India. Asian Journal of Natural Product Biochemistry, 20 (2): doi:10.13057/biofar/f200202

Itoh, K., Matsukawa, T., Okamoto, M., Minami, K., Tomohiro, N., Shimizu, K., Kajiyama, S., Endo, Y., Matsuda, H. and Shigeoka, S. (2020). In vitro antioxidant activity of Mangifera indica leaf extracts. Journal of Plant Studies, 9(2): 39. doi:10.5539/jps. v9n2p39.

Karigidi, M.E., Fakunle, O.E. and Karigidi, K.O. (2025). Antioxidant, antidiabetic and antifungal activities of leaf and bark ethanol extracts of Mangifera indica and their antagonistic biochemical effects. International Journal of Functional Nutrition, 6 (1):1–11. doi:10.3892/ijfn.2025.46. Spandidos Publications.

Khorrami, S., Zarrabi, A., Khaleghi, M., Danaei, M. and Mozafari, M. (2018). Selective cytotoxicity of green synthesized silver nanoparticles against the MCF-7 tumor cell line and their enhanced antioxidant and antimicrobial properties. International Journal of Nanomedicine, 13: 8013–8024. doi:10.2147/IJN.S189295.

Kshama, Soumya, V. and Ray, N. M. (2025). Phytochemical characterization and radical scavenging activity of Roylea cinerea leaves. Research Journal of Biotechnology, 8(20):108. doi:10.25303/208rjbt1080119. World Researchers Associations.

Kumar, M., Saurabh, V., Tomar, M., Hasan, M., Changan, S., Sasi, M., Maheshwari, C., Prajapati, U., Singh, S. and Prajapat, R.K., Dhumal, S., Punia, S., Amarowicz, R. and Mekhemar, M. (2021). Mango (Mangifera indica L.) leaves: nutritional composition, phytochemical profile, and health-promoting bioactivities. Antioxidants, 10(2):299. doi:10.3390/ antiox10020299.

Maharaj, A., Naidoo, Y., Dewir, Y.H. and Rihan, H. (2022). Phytochemical screening, and antibacterial and antioxidant activities of Mangifera indica leaves. Horticulturae, 8 (10) : 909. doi:10.3390/horticulturae8100909. Multidisciplinary Digital Publishing Institute.

Muralikrishna, T., Malothu, R., Pattanayak, M. and Nayak, P.L. (2014). Green synthesis of gold nanoparticles using Mangifera indica (Mango Leaves) aqueous extract. World Journal of Nano Science and Technology, 3(2): 66-73. doi:10.5829/idosi.wjnst.2014.3.2.114.

Ogidi, O., Okore, C., Akpan, U., Ayebabogha, M. and Onukwufo, C. (2021). Evaluation of antimicrobial activity and bioactive phytochemical properties of mango (Mangifera indica) stem- bark extracts. International Journal of Pharmacognosy, 8(5): 189–195. doi:10.13040/IJPSR.0975-8232.

Pan, J., Yi, X., Zhang, S., Cheng, J., Wang, Y., Liu, C. and He, X. (2018). Bioactive phenolics from mango leaves (Mangifera indica L.). Industrial Crops and Products, 111:400–406. doi:10.1016/j.indcrop.2017.10.057.

Samari, F., Salehipoor, H., Eftekhar, E. and Yousefinejad, S. (2018). Low- temperature biosynthesis of silver nanoparticles using mango leaf extract: catalytic effect, antioxidant properties, anticancer activity and application for colorimetric sensing. New Journal of Chemistry, 42(19: T15905–15916. doi:10.1039/C8NJ03156H. he Royal Society of Chemistry.

Somkuwar, D.O. and Kamble, V.A. (2013). Phytochemical screening of ethanolic extracts of stem, leaves, flower and seed kernel of Mangifera indica L. International Journal of Pharma and Bio Sciences, 4: 383–389.

Sreelatha, R., Kasturi, A.P.K. and Challa, M.M. (2025). Gas chromatography- mass spectroscopy profiling of leaf extracts of Stachytarpheta urticifolia (Salisb) based on antioxidant activities. Research Journal of Biotechnology, 20(7): 91–97. doi:10.25303/207rjbt091097. World Researchers Associations.

Downloads

Submitted

07-10-2025

Published

13-10-2025

How to Cite

I. Manikkavasagan, K. Vijayarani, B. Murugan, S. Meignanalakshmi, S. Eswari, & V. Ragavendiran. (2025). PHYTOCHEMICAL CHARACTERIZATION AND EVALUATION OF ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF MANGIFERA INDICA LEAF EXTRACT. Indian Journal of Veterinary and Animal Sciences Research, 54(5), 57-67. https://doi.org/10.56093/ijvasr.v54i5.172412
Citation