MICROALGAE AS MULTIFUNCTIONAL FEED ADDITIVES IN POULTRY: ROLES IN NUTRITION, HEALTH AND PRODUCT QUALITY
128 / 184
Keywords:
Microalgae, Poultry nutrition, Antioxidant, Immune modulation, Gut health, Egg and meat qualityAbstract
Microalgae have gained increasing attention as multifunctional feed ingredients in poultry nutrition due to their rich content of bioactive compounds, including pigments, polyunsaturated fatty acids, vitamins, minerals, polysaccharides, and secondary metabolites. This review summarizes experimental evidence on the functional properties of major microalgal species such as Spirulina (Arthrospira platensis), Chlorella spp., Haematococcuspluvialis, Nannochloropsis, Schizochytrium, and other marine microalgae in poultry diets. Emphasis is placed on their antioxidant, antimicrobial, anti-inflammatory, immunomodulatory, and gut healthpromoting effects. Microalgal supplementation enhances endogenous antioxidant defence systems, modulates cytokine responses, suppresses enteric pathogens, and improves intestinal morphology and microbiota composition. These functional effects translate into improved stress tolerance, immune competence, nutrient utilization, and overall bird health. In addition, microalgae positively influence poultry product quality by enhancing yolk pigmentation, enriching eggs and meat with omega-3 fatty acids, and improving oxidative stability and sensory attributes. Variability in responses across studies is largely associated with differences in algal species, inclusion level, processing form, duration of feeding, and physiological status of birds. Overall, microalgae represent promising natural feed additives for sustainable poultry production, although further research is needed to optimize their practical application at commercial scale.
Downloads
References
Abdel-Daim, M.M., Abuzead, S.M.M. and Halawa, S.M. (2013). Protective role of Spirulina platensis against acute deltamethrin-induced toxicity in rats. PLOS ONE, 8: e72991.
Abdel-Moneim, A.M.E., Shehata, A.M., Mohamed, N.G., Elbaz, A.M. and Ibrahim, N.S. (2022). Synergistic effect of Spirulina platensis and selenium nanoparticles on growth performance, serum metabolites, immune responses, and antioxidant capacity of heat-stressed broiler chickens. Biological Trace Element Research, 200: 768–779.
Abdelnour, S.A., Abd El-Hack, M.E., Arif, M., Khafaga, A.F. and Taha, A.E. (2019). The application of the microalgae Chlorella spp. as a supplement in broiler feed. World’s Poultry Science Journal, 75: 305–318.
Abdel-Wareth, A.A.A., Williams, A.N., Salahuddin, M., Gadekar, S. and Lohakare, J. (2024). Algae as an alternative source of protein in poultry diets for sustainable production and disease resistance: present status and future considerations. Frontiers in Veterinary Science, 11: 1382163.
Abou-Gabal, A., Aboul-Ela, H.M., Ali, E., Ahmed, E. and Shalaby, O.K. (2015). Hepatoprotective, DNA damage prevention and antioxidant potential of Spirulina platensis on CCl₄-induced hepatotoxicity in mice. American Journal of Biomedical Research, 3: 29–34.
Al-Batshan, H.A., Al-Mufarrej, S.I., Homaidan, A.A. and Qureshi, M.A. (2001). Enhancement of chicken macrophage phagocytic function and nitrite production by dietary Spirulina platensis. Immunopharmacology and Immunotoxicology, 23: 281–289.
Alghamdi, M.A., Elbaz, M.I., Ismail, I.E., Reda, F.M., Alagawany, M., El- Tarabily, K.A. and Abdelgeliel, A.S. (2024). Dietary supplementation with a mixture of Dunaliella salina and Spirulina enhances broiler performance by improving growth, immunity, digestive enzymes and gut microbiota. Poultry Science, 103: 103337.
An, B.K., Kim, K.E., Jeon, J.Y. and Lee, K.W. (2016). Effect of dried Chlorella vulgaris and chlorella growth factor on growth performance, meat qualities and humoral immune responses in broiler chickens. SpringerPlus, 5(1): 718.
Ávila-Román, J., García-Gil, S., Rodríguez- Luna, A., Motilva, V. and Talero, E. (2021). Anti-inflammatory and anticancer effects of microalgal carotenoids. Marine Drugs, 19: 531.
Balakrishnan, J., Ganapathi, P., Kannan, S., Marudhamuthu, M. and Shanmugam, K. (2021). Anti-listerial activity of microalgal fatty acid methyl esters and their possible applications as chicken marinade. International Journal of Food Microbiology, 339: 109027.
Bonos, E., Kasapidou, E., Kargopoulos, A., Karampampas, A., Christaki, E., Florou-Paneri, P. and Nikolakakis, I. (2016). Spirulina as a functional ingredient in broiler chicken diets. South African Journal of Animal Science, 46: 94–102.
Chang, C.W.T., Takemoto, J.Y., Chang, P.E., AlFindee, M.N. and Lin, Y.Y. (2021). Effects of mesobiliverdin IXα- enriched microalgae feed on gut health and microbiota of broilers. Frontiers in Veterinary Science, 7: 586813.
Chaudhary, A., Mishra, P., Al Amaz, S., Mahato, P.L., Das, R., Jha, R. and Mishra, B. (2023). Dietary supplementation of microalgae mitigates the negative effects of heat stress in broilers. Poultry Science, 102: 102958.
Choo, W.T., Teoh, M.L., Phang, S.M., Convey, P., Yap, W.H., Goh, B.H. and Beardall, J. (2020). Microalgae as potential anti-inflammatory natural product against human inflammatory skin diseases. Frontiers in Pharmacology, 11: 1086.
Corrales-Martínez, J., Ortega-Paredes, D., Šefcová, M.A., Larrea-Álvarez, C.M., de Janon, S., Medina-Santana, J., Molina-Cuasapaz, G., Vinueza-Burgos, C., Revajová, V., Larrea- Álvarez, M. and Calero-Cáceres, W. (2022). A PMAxx™ qPCR assay reveals that dietary administration of the microalgae Tetraselmis chuii does not affect Salmonella infantis caecal content in early-treated broiler chickens. Veterinary Sciences, 9(9): 487.
Dal Bosco, A., Gerencsér, Z., Szendrő, Z., Mugnai, C., Cullere, M., Kovács, M., Ruggeri, S., Mattioli, S. and DalleZotte, A. (2014). Effect of dietary spirulina and thyme on rabbit meat appearance, oxidative stability and fatty acid profile during retail display. Meat Science, 96: 114–119.
El-Bahr, S.M., Shousha, S., Shehab, A., Albaqami, N.M., Aboul-Soud, M.A.M., Soliman, M.M. and Ghonim, A. (2020). Effect of dietary microalgae on growth performance, profiles of amino and fatty acids, antioxidant status, and meat quality of broiler chickens. Animals, 10: 761.
El-Baz, F.K., El-Senousy, W.M., El-Sayed, A.B. and Kamel, M.M. (2013). In vitro antiviral and antimicrobial activities of Spirulina platensis extract. Journal of Applied Pharmaceutical Science, 3: 52–56.
El-Ghany, W.A.A. (2020). Microalgae in poultry field: a comprehensive perspectives. Advances in Animal and Veterinary Sciences, 8(9): 888–897.
El-Sheekh, M.M., Daboor, S.M., Swelim, M.A. and Mohamed, S. (2014). Production and characterization of antimicrobial active substance from Spirulina platensis. Iranian Journal of Microbiology, 6: 112–119.
Emam, K.R.S., Ali, S.A.M., Morsy, A.S., Fouda, W.A. and Elbaz, A.M. (2024). Role of Nannochloropsis oculata supplement in improving performance, antioxidant status, blood metabolites, and egg quality of laying hens under hot environmental conditions. Scientific Reports, 14: 16884.
Englmaierová, M., Škrivan, M. and Bubancová, I. (2013). A comparison of lutein, spray-dried chlorella, and synthetic carotenoids on yolk colour, oxidative stability, and reproductive performance of laying hens. Czech Journal of Animal Science, 58: 412–419.
Fathi, M.A., Namra, M.M.M., Ragab, M.S. and Aly, M.M.M. (2018). Effect of dietary supplementation of algae meal (Spirulina platensis) on performance of broiler chickens. Egyptian Poultry Science Journal, 38: 375–389.
Fernandes, R.T.V., Gonçalves, A.A. and de Arruda, A.M.V. (2020). Production, egg quality, and intestinal morphometry of laying hens fed marine microalga. Revista Brasileira de Zootecnia, 49: e20200011.
Ferrazzano, G.F., Papa, C., Pollio, A., Ingenito, A., Sangianantoni, G. and Cantile, T. (2020). Cyanobacteria and microalgae as sources of functional foods to improve human general and oral health. Molecules, 25: 5164.
Janczyk, P., Halle, B. and Souffrant, W.B. (2009). Microbial community composition of the crop and ceca contents of laying hens fed diets supplemented with Chlorella vulgaris. Poultry Science, 88: 2324–2332.
Kang, H.K., Salim, H.M., Akter, N., Kim, D.W., Kim, J.H., Bang, H.T., Kim, M.J., Na, J.C., Hwangbo, J., Choi, H.C. and Suh, O.S. (2013). Effect of various forms of dietary chlorella supplementation on growth performance, immune characteristics, and intestinal microflora population of broiler chickens. Journal of Applied Poultry Research, 22(1): 100–108.
Kaushik, P. and Chauhan, A. (2008). In vitro antibacterial activity of laboratory grown culture of Spirulina platensis. Indian Journal of Microbiology, 48:348–352.
Khalilnia, F., Mottaghitalab, M., Mohiti, R. and Seighalani, R. (2023). Effects of dietary supplementation of probiotic and Spirulina platensis microalgae powder on growth performance, immune response, carcass characteristics, gastrointestinal microflora and meat quality in broiler chicks. Veterinary Medicine and Science, 9: 1666–1674.
Kulshreshtha, G., Rathgeber, B., Stratton, G., Thomas, N., Evans, F., Critchley, A., Hafting, J. and Prithiviraj, B. (2014). Feed supplementation with red seaweeds Chondrus crispus and Sarcodiotheca gaudichaudii affects performance, egg quality, and gut microbiota of layer hens. Poultry Science, 93: 2991–3001.
Lee, J.Y., Yoon, J.H., An, S.H., Cho, I.H., Lee, C.W., Jeon, Y.J., Joo, S.S., Ban, B.C., Lee, J.-Y., Jung, H.J., Kim, M., Kim, Z.-H., Jung, J.Y., Kim, M. and Kong, C. (2023). Intestinal immune cell populations, barrier function, and microbiomes in broilers fed a diet supplemented with Chlorella vulgaris. Animals, 13(14): 2380.
Levine, R., Horst, G., Tonda, R., Lumpkins, B. and Mathis, G. (2018). Evaluation of the effects of feeding dried algae containing beta-1,3-glucan on broilers challenged with Eimeria. Poultry Science, 97: 3594–3600.
Liu, L., Li, Q., Yang, Y. and Guo, A. (2021). Biological function of short-chain fatty acids and its regulation on intestinal health of poultry. Frontiers in Veterinary Science, 8: 736739.
Long, S.F., Kang, S., Wang, Q.Q., Xu, Y.T., Pan, L., Hu, J.X., Li, M. and Piao, X.S. (2018). Dietary supplementation with DHA-rich microalgae improves performance, serum composition, carcass traits, antioxidant status, and fatty acid profile of broilers. Poultry Science, 97: 1881–1890.
Mala, R., Sarojini, M., Saravanababu, S. and Umadevi, G. (2009). Screening for antimicrobial activity of crude extracts of Spirulina platensis. Journal of Cell and Tissue Research, 9: 1951–1955.
Mariey, Y.A., Samak, H.R. and Ibrahem, M.A. (2012). Effect of using Spirulina platensis algae as a feed additive for poultry diets: Productive and reproductive performance of local laying hens. Egyptian Poultry Science Journal, 32: 201–215.
Mens, A.J.W., van Krimpen, M.M., Kar, S.K., Guiscafre, F.J. and Sijtsma, L. (2022). Enriching table eggs with n-3 polyunsaturated fatty acids through dietary supplementation with the phototrophically grown green algae Nannochloropsis limnetica: Effects of microalgae on nutrient retention, performance, egg characteristics and health parameters. Poultry Science, 101: 101869.
Mirzaie, S., Zirak-Khattab, F., Hosseini, S.A. and Donyaei-Darian, H. (2018). Effects of dietary Spirulina on antioxidant status, lipid profile, immune response and performance characteristics of broiler chickens reared under high ambient temperature. Asian-Australasian Journal of Animal Sciences, 31(4): 556–563.
Mobarez, S.M., Rizk, A.M., Abdel Latif, A.M. and Osama, A.E. (2018). Effect of supplementing diet with Spirulina platensis algae or turmeric on productive and reproductive performance of Golden Montazah layers. Egyptian Poultry Science Journal, 38: 109–125.
Moradikor, N. and Mohamadi, N. (2015). The effects of different levels of chlorella microalgae on performance and immune response of laying hens under heat stress condition. International Journal of Life Sciences, 9: 71–74.
Nabil-Adam, A., Ashour, M.L. and Shreadah, M.A. (2023). Modulation of MAPK/NF-κB pathway and NLRP3 inflammasome by secondary metabolites from red algae: A mechanistic study. ACS Omega, 8: 37971–37990.
Novoveská, L., Nielsen, S.L., Eroldoğan, O.T., Haznedaroglu, B.Z., Rinkevich, B., Fazi, S., Robbens, J. and Einarsson, H. (2023). Overview and challenges of large-scale cultivation of photosynthetic microalgae and cyanobacteria. Marine Drugs, 21: 445.
Nuhu, A.A. (2013).Spirulina (Arthrospira): An important source of nutritional and medicinal compounds. Journal of Marine Biology, 1: 1–8.
Oh, S.T., Zheng, L., Kwon, H.J., Choo, Y.K., Lee, K.W., Kang, C.W. and An, B.K. (2015). Effects of dietary fermented Chlorella vulgaris (CBT®) on growth performance, relative organ weights, cecal microflora, tibia bone characteristics, and meat qualities in Pekin ducks. Asian-Australasian Journal of Animal Sciences, 28: 95–101.
Panaite, T.D., Cornescu, G.M., Predescu, N.C., Cismileanu, A., Turcu, R.P., Șaracila, M. and Soica, C. (2023). Microalgae (Chlorella vulgaris and Spirulina platensis) as a protein alternative and their effects on productive performances, blood parameters, protein digestibility, and nutritional value of laying hens’ egg. Applied Sciences, 13(18): 10451.
Park, J.H., Lee, S.I. and Kim, I.H. (2018). Effect of dietary Spirulina (Arthrospira platensis) on growth performance, antioxidant enzyme activity, nutrient digestibility, cecal microflora, excreta noxious gas emission and breast meat quality of broiler chickens. Poultry Science, 97: 2451–2459.
Parpinello, G.P., Meluzzi, A., Sirri, F., Tallarico, N. and Versri, A. (2006). Sensory evaluation of egg products and eggs laid from hens fed diets with different fatty acid composition and supplemented with antioxidants. Food Research International, 39: 47–52.
Pugh, N., Ross, S.A., Elsohly, H.N., Elsohly, M.A. and Pasco, D.S. (2001). Isolation of three high molecular weight polysaccharide preparations with potent immunostimulatory activity from Spirulina platensis, Aphanizomenon flos-aquae and Chlorella pyrenoidosa. Planta Medica, 67: 737–742.
Qureshi, M.A., Garlich, J.D. and Kidd, M.T. (1996). Dietary Spirulina platensis enhances humoral and cell-mediated immune functions in chickens. Immunopharmacology and Immunotoxicology, 18: 465–476.
Raju, M.V.L.N., Rao, S.V., Radhika, K. and Chawak, M.M. (2005). Dietary supplementation of spirulina and its effects on broiler chicken exposed to aflatoxicosis. Indian Journal of Poultry Science, 40: 36–40.
Rizzi, L., Bochicchio, D., Bargellini, A., Parazza, P. and Simioli, M. (2009). Effects of dietary microalgae and other lipid sources on egg yolk n-3 fatty acid composition, selenium content and quality of eggs in laying hens. Journal of the Science of Food and Agriculture, 89: 1775–1781.
Rojas, V., Rivas, L., Cárdenas, C. and Guzmán, F. (2020). Cyanobacteria and eukaryotic microalgae as emerging sources of antibacterial peptides. Molecules, 25: 5804.
Šefcová, M.A., Santacruz, F., Larrea- Álvarez, C.M., Vinueza-Burgos, C., Ortega-Paredes, D., Molina-Cuasapaz, G., Rodríguez, J., Calero-Cáceres, W., Revajová, V., Fernández-Moreira, E. and Larrea-Álvarez, M. (2021). Administration of dietary microalgae ameliorates intestinal parameters, improves body weight, and reduces thawing loss of fillets in broiler chickens: A pilot study. Animals, 11(12): 3601.
Shanmugapriya, B., Babu, S.S., Hariharan, T., Sivaneswaran, S. and Anusha, M.B. (2015). Dietary administration of Spirulina platensis as probiotics on growth performance and histopathology in broiler chicks. International Journal of Recent Scientific Research, 6: 2650–2653.
Stirk, W.A. and van Staden, J. (2022). Bioprospecting for bioactive compounds in microalgae: Antimicrobial compounds. Biotechnology Advances, 59: 107977.
Talero, E., García-Mauriño, S., Ávila- Román, J., Rodríguez-Luna, A., Alcaide, A. and Motilva, V. (2015). Bioactive compounds isolated from microalgae in chronic inflammation and cancer. Marine Drugs, 13: 6152–6209.
Tsuchihashi, N., Watanabe, T. and Takai, Y. (1987). Effect of Spirulina platensis on caecum content in rats. Bulletin of Chiba Hygienic College, 7: 27–30.
Van Nerom, S., Coleman, B., De Baets, R., Van Immerseel, F., Robbens, J. and Delezie, E. (2024). Microalgae as feed additives in poultry: A review on the health-promoting effects. Algal Research, 83: 103733.
Waldenstedt, L., Inborr, J., Hansson, I. and Elwinger, K. (2003). Effects of astaxanthin-rich algal meal (Haematococcus pluvialis) on growth performance, caecal Campylobacter and Clostridium counts, and tissue astaxanthin concentration of broiler chickens. Animal Feed Science and Technology, 108: 119–132.
Yusuf, M.S., Hassan, M.A., Abdel-Daim, M.M., El-Nabtiti, A.S., Ahmed, A.M., Moawed, S.A., El-Sayed, A.K. and Cui, H. (2016). Value added by Spirulina platensis in two different diets on growth performance, gut microbiota and meat quality of Japanese quails. Veterinary World, 9:1287–1293.
Zeweil, H., Abaza, I.M., Zahran, S.M., Ahmed, M.H., Haiam, M., Aboul- Ela, A.S. and Asmaa, A.S. (2016). Effect of Spirulina platensis as dietary supplement on some biological traits for chickens under heat stress condition. Asian Journal of Biomedical and Pharmaceutical Sciences, 6: 8–12.
Zhang, L., Jiang, Y., Buzdar, J.A., Ahmed, S., Sun, X., Li, F., Ma, L., Wu, P.F. and Li, C. (2025). Microalgae: an exciting alternative protein source and nutraceutical for the poultry sector. Food Science of Animal Resources, 45(1): 243–265.
Downloads
Submitted
Published
Issue
Section
License
All the copy right belongs to the sponsoring Organization, Tamil Nadu Veterinary and Animal Sciences University, Chennai - 51.