QUALITY AND SAFETY EVALUATION OF CHICKEN SHAWARMA IN CHENNAI
56 / 45
Keywords:
Shawarma, rancidity profile, histamine, microbial contaminationAbstract
Shawarma is a popular ready-to-eat food consumed by a diverse spectrum of populations in Chennai and other parts of India. The consumption of shawarma has dramatically increased in recent years. Although it is consumed by many people, especially the younger generation, the quality and safety of the shawarma are questionable. In this study, 10 chicken shawarma samples were purchased from local restaurants in Chennai city to assess their quality and safety by estimating rancidity profile in terms of free fatty acid (FFA) and peroxide value, histamine content, and microbial contamination. The results revealed that the percentage of FFA and peroxide values ranged from 1.41±0.99 to 7.05±1.73 and 2±0.57 to 14±1.47 meq/kg, respectively. Among the tested samples, 30% showed the presence of a significant level of histamine content (1.7–7 mg/100g). The biochemical characterization of bacterial isolates from shawarma samples revealed the presence of contamination by Salmonella enteric sub sp. enterica, Escherichia coli STEC 367, Listeria monocytogenes, and Listeria innocua. Further, these isolates were also confirmed by PCR by amplifying a 284 bp invA gene fragment for Salmonella sp., an 884 bp uspA gene fragment for E. coli, and a 457 bp iap gene fragment for Listeria sp. The findings of this study emphasize the significance of safety monitoring for ready-to-eat foods.
Downloads
References
Abdelhai, M.H., Sulieman, A.M.E. and Babiker, E.R.B. (2015). Some chemical and microbiological characteristics of shawerma meat product. Journal of Food and Nutritional Disorders, 4: 2.
Ahmad, M.U.D., Sarwar, A., Najeeb, M.I., Nawaz, M., Anjum, A.A., Ali, M.A. and Mansur, N. (2013). Assessment of microbial load of raw meat at abattoirs and retail outlets. The Journal of Animal and Plant Sciences, 23(3): 745-748.
Ahmed Ali Massad, F. and Adam Mariod, A. (2021). Peroxide value in oils used for chicken frying in selected restaurants in Khartoum city- Khartoum State- Sudan. Europian Journal of Food Science and Technology, 5(1): 6-10.
Aidara-Kane, A., Angulo, F.J., Conly, J.M., Minato, Y., Silbergeld, E.K., McEwen, S.A. and Collignon, P.J. (2018). World Health Organization (WHO) guidelines on use of medically important antimicrobials in food- producing animals. Antimicrobial Resistance and Infection Control, 7: 7.
Akbar, A. and Anal, A.K. (2015). Isolation of Salmonella from ready-to-eat poultry meat and evaluation of its survival at low temperature, microwaving and simulated gastric fluid. Journal of Food Science and Technology, 52(5): 3051-3057.
Aktar, N., Bilkis, R. and Ilias, M. (2016). Isolation and identification of Salmonella sp. from different food. International Journal of Biosciences, 8(2): 16-24.
Al-Brefkani, A.M.T. and Mammani, I.M.A. (2019). Characterisation of Listeria monocytogenes from food and human clinical samples at Duhok, Kurdistan region of Iraq. Journal of Pure and Applied Microbiology, 13(4): 2215- 2226.
Alharbi, S.A., Abdel-Ghaffar, M.H. and Nivas, K. (2019). Isolation and identification of pathogenic bacteria from ready-to-eat fast foods in Al- Quwayiyah, Kingdom of Saudi Arabia. African Journal of Food, Agriculture, Nutrition and Development, 19(3): 14739-14751.
Ashrafudoulla, M., Na, K.W., Byun, K.H., Kim, D.H., Yoon, J.W., Mizan, F.R., Kang, I. and Ha, S. (2021). Isolation and characterization of Salmonella spp. from food and food contact surfaces in a chicken processing factory. Poultry Science, 100: 101234.
Bailey, W.R. and Scott, E.G. (1998). Diagnostic Microbiology. Textbook for isolation and identification of pathogenic organisms. The C.V Mosby Company Sarnt Houis.
Biswas, S., Parvez, M.A.K., Shafiquzzaman, M., Nahar, S. and Rahman, M.N. (2010). Isolation and characterization of Escherichia coli in ready-to-eat foods vended in Islamic University, Kushtia. Journal of Biosciences, 18: 99-103.
Cocolin, L., Rantsiou, K., Iacumin, L., Cantoni, C. and Comi, G. (2002). Direct identification in food samples of Listeria spp. and Listeria monocytogenes by molecular methods. Applied and Environmental Microbiology, 68(12): 6273-6282.
El-Shenawy,M., El-Shenawy, M., Manes, J. and Soriano, J.M. (2011). Listeria spp. in street-vended ready- to-eat foods. Interdisciplinary Perspectives on Infectious Diseases, Hindawi Publishing Corporation, Article ID 968031, 6 pages (doi:10.1155/2011/968031).
Ema, F.A., Shanta, R.N., Rahman, M.Z., Islam, M.A. and Khatun, M.M. (2022). Isolation, identification, and antibiogram studies of Escherichia coli from ready-to-eat foods in Mymensingh, Bangladesh. Veterinary World, 15(6): 1497-1505.
FAO: Food and Agriculture Organization 1992. Manual of Food Quality Control. Food and Agriculture Organization, Part (4), Microbiological examination, United Nations, Rome.
Fatima, K., Khan, A. and Jadoon, A. (2024). Isolation and biochemical characterization of food-borne Listeria spp from Haelian. Journal of Population Therapeutics and Clinical Pharmacology, 31(6): 1346-1352.
Giri, S., Kudva, V., Shetty, K. and Shetty, V. (2021). Prevalence and characterization of extended spectrum lactamase producing antibiotic resistant Escherichia coli and Klebsiella pneumoniae in Ready-to- Eat Street Foods. Antibiotics, 10: 850.
Goudar, V., Kanthesh, B.M. and Prasad, N. (2021). Isolation and identification of Listeria monocytogenes in Bangalore city from various food samples. Biomedical and Pharmacology Journal, 14(4): 2271-2276.
Mariana, R.R., Susanti, E., Hidayati, L. and Wahab, R.A. (2020). Analysis of peroxide value, free fatty acid, and water content changes in used cooking oil from street vendors in Malang. AIP Conference Proceedings, 2231 -040057.
Nayak, D.N., Savalia1, C.V., Kalyani, I.H., Kumar, R. and Kshirsagar, D.P. (2015). Isolation, identification, and characterization of Listeria spp. from various animal origin foods. Veterinary World, 8: 2231-0916.
Odu, N.N. and Akano, U.M. (2012). Microbiological Assessment of Ready-To-Eat-Food (Shawerma) In Port Harcourt City, Nigeria. Nature and Science, 10(8): 1-8.
Razaa, J., Asmata, T.M., Mustafaa, M.Z., Ishtiaqc, H., Mumtazd, K., Jaleese, M.M., Samada, A., Shaha, A.A., Khalida, S. and Rehman, H. (2021). Contamination of ready-to-eat street food in Pakistan with Salmonella spp.: Implications for consumers and food safety, International Journal of Infectious Diseases, 106: 123-127.
Salem, N.I.E., El Gamel, A.M., Khalifa, A.M.A., Abd-El-Hady, H.A.M. and Abou Zeid, M.A.M. (2016). Microbial status of shawerma sandwiches in Kafr El-Sheikh Governorate. Alexandria Journal of Veterinary Sciences, 51(2): 303-309.
Sallam, K.I., Morgan, S.E.M., Sayed- Ahmed, M.Z., Alqahtani, S.S. and Abd-Elghany, S.M. (2021). Health hazard from exposure to histamine produced in ready-to-eat shawarma widely consumed in Egypt. Journal of Food Composition and Analysis, 97: 103794.
Tarabees, R., Elsayed, M.S.A., Shawish, R., Basiouni, S. and Shehata, A.A. (2017). Isolation and characterization of Salmonella enteritidis and Salmonella typhimurium from chicken meat in Egypt. Journal of Infectious Diseases, 11(4): 314-319.
Weichselbaum, E., Coe, S., Buttriss, J. and Stanner, S. (2013). Fish in the diet: A review. Nutrition Bulletin, 38(2): 128-177.
Yang, G., Zhang, S., Huang, Y., Ye, Q., Zhang, J., Wu, Q., Wang, J., Chen, M. and Xue, L. (2020). Isolation and characterization of non-O157 shiga toxin–producing Escherichia coli in foods sold at retail markets in China. Journal of Food Protection, 83(3): 460-466.
Downloads
Submitted
Published
Issue
Section
License
All the copy right belongs to the sponsoring Organization, Tamil Nadu Veterinary and Animal Sciences University, Chennai - 51.